Biocompatibility of Ferrara intracorneal ring segment with and without chondroitin sulfate coating: Clinical and histopathological evaluation in rabbits


Autoria(s): Andreghetti, Eduardo; Hashimoto, Miriam; Domingues, Maria Aparecida Custódio; Antunes, Vitor Andrigheti Coronado; Segundo, Paulo de Souza; Silva, Maria Rosa Bet de Moraes
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

01/09/2013

Resumo

PURPOSE: To investigate and compare the biocompatibility of two types of Ferrara intracorneal ring segment: with and without chondroitin sulfate coating by clinical and histopathological evaluation. METHODS: A randomized experimental study was carried out on thirty right-eye corneas from 30 Norfolk albino rabbits allocated into two experimental groups: Group G1 - implanted with Ferrara intracorneal ring segment without coating (FICRS) and Group G2 - implanted with Ferrara intracorneal ring segment with chondroitin sulfate coating (FICRS-CS). Left eyes formed the control group. Clinical parameters analyzed were: presence of edema, vascularization, infection and ring extrusion one, 30, and 60 days after surgery. Histopathological parameters analyzed were: number of corneal epithelial layers over and adjacent to the ring, presence of spongiosis, hydropic degeneration, basement membrane thinning, inflammatory cells, neovascularization and pseudocapsule formation. RESULTS: At clinical examination 60 days after implant, edema, vascularization and extrusion were observed respectively in 20%, 26.7%, 6.7% of FICRS corneas and in 6.7%, 6.7%, and 0% of FICRS-CS corneas. Histopathological evaluation showed epithelial-layer reduction from 5 (5;6) to 3 (3;3) with FICRS and from 5 (5;5) to 4 (3;5) with FICRS-CS in the region over the ring. Epithelial spongiosis, hydropic degeneration, and basement membrane thinning were present in 69.2%, 53.8%, and 69.2% of FICRS and in 73.3%, 73.3%, and 46.7% with FICRS-CS, respectively. Vascularization was present in 38.5% of FICRS and 13.3% with FICRS-CS, inflammatory cells in 75% of FICRS and 33.3% with FICRS-CS, and pseudocapsule in 66.7% of FICRS and 93.3% with FICRS-CS. Giant cells occurred only in the FICRS-CS group (20%). CONCLUSION: Ferrara intracorneal rings coated with chondroitin sulfate (FICRS-CS) caused lower frequency of clinical and histopathological alterations than Ferrara intracorneal rings without the coating (FICRS), demonstrating higher biocompatibility of the FICRS-CS.

Formato

632-640

Identificador

http://dx.doi.org/10.1590/S0102-86502013000900002

Acta Cirurgica Brasileira, v. 28, n. 9, p. 632-640, 2013.

0102-8650

1678-2674

http://hdl.handle.net/11449/76466

10.1590/S0102-86502013000900002

S0102-86502013000900002

WOS:000324216400002

2-s2.0-84883645775

2-s2.0-84883645775.pdf

Idioma(s)

eng

Relação

Acta Cirúrgica Brasileira

Direitos

openAccess

Palavras-Chave #Chondroitin sulfates #Cornea #Corneal neovascularization #Prosthesis implantation #Rabbits #chondroitin sulfate #animal experiment #animal tissue #biocompatibility #controlled study #cornea neovascularization #corneal pachymetry #edema #epithelium cell #female #giant cell #histopathology #inflammatory cell #intrastromal corneal ring segment #nonhuman #postoperative complication #rabbit
Tipo

info:eu-repo/semantics/article