Bifunctional silica nanoparticles for the exploration of biofilms of Pseudomonas aeruginosa
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
27/05/2014
27/05/2014
01/08/2013
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Resumo |
Luminescent silica nanoparticles are frequently employed for biotechnology applications mainly because of their easy functionalization, photo-stability, and biocompatibility. Bifunctional silica nanoparticles (BSNPs) are described here as new efficient tools for investigating complex biological systems such as biofilms. Photoluminescence is brought about by the incorporation of a silylated ruthenium(II) complex. The surface properties of the silica particles were designed by reaction with amino-organosilanes, quaternary ammonium-organosilanes, carboxylate-organosilanes and hexamethyldisilazane. BSNPs were characterized extensively by DRIFT, 13C and 29Si solid state NMR, XPS, and photoluminescence. Zeta potential and contact angle measurements exhibited various surface properties (hydrophilic/hydrophobic balance and electric charge) according to the functional groups. Confocal laser scanning microscopy (CLSM) measurements showed that the spatial distribution of these nanoparticles inside a biofilm of Pseudomonas aeruginosa PAO1 depends more on their hydrophilic/hydrophobic characteristics than on their size. CLSM observations using two nanosized particles (25 and 68 nm) suggest that narrow diffusion paths exist through the extracellular polymeric substances matrix. © 2013 Copyright Taylor and Francis Group, LLC. |
Formato |
775-788 |
Identificador |
http://dx.doi.org/10.1080/08927014.2013.798866 Biofouling, v. 29, n. 7, p. 775-788, 2013. 0892-7014 1029-2454 http://hdl.handle.net/11449/76147 10.1080/08927014.2013.798866 WOS:000321828200003 2-s2.0-84880508754 |
Idioma(s) |
eng |
Relação |
Biofouling |
Direitos |
closedAccess |
Palavras-Chave | #bifunctional silica nanoparticles #P. aeruginosa (PAO1) biofilms #photoluminescence #surface modification #biofilm #biotechnology #diffusion #functional group #luminescence #microbial community #microscopy #nanotechnology #particle size #polymer #reaction kinetics #ruthenium #silica #Pseudomonas aeruginosa |
Tipo |
info:eu-repo/semantics/article |