Lack of reliability of nanotechnology in the of free plasma DNA in samples of patients with prostate cancer


Autoria(s): Moreno, Ricardo; Delgado, Pamela O; Coelho, Patrícia G; Marsicano, Sarah R; Boas, Viviane Av; Azzalis, Ligia A; Junqueira, Virgínia Bc; Rocha, Katya C; De Abreu, Luiz Carlos; Valenti, Vitor E; Drezzet, Jefferson; Pereira, Edimar Cristiano; Fonseca, Fernando La
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

16/01/2013

Resumo

Background: Several studies seek biological markers that give diagnostic and degree of tumor development. The aim of this study was to validate the determination of plasma DNA using nanotechnology (Nanovue™-NV) in samples of 80 patients with prostate cancer. Methods. Blood samples of 80 patients of the Urology Ambulatory of Faculdade de Medicina do ABC with prostate cancer confirmed by anatomical-pathology criteria were analyzed. DNA extraction was performed using a GFX TM kit (Amersham Pharmacia Biotech, Inc, USA) following the adapted protocol. Plasma was subjected to centrifugation. Results: There was a big difference between the first and the second value obtained by NanoVue Only two samples had no differences between duplicates. Maximum difference between duplicates was 38 μg/mL. Average variation between 51 samples was 10.29 μg/mL, although 21 samples had differences above this average. No correlation was observed between pDNA obtained by traditional spectrophotometry and by nanotechnology. Conclusion: Determination of plasma DNA by nanotechnology was not reproducible. © 2013 Moreno et al; licensee BioMed Central Ltd.

Identificador

http://dx.doi.org/10.1186/1755-7682-6-2

International Archives of Medicine, v. 6, n. 1, 2013.

1755-7682

http://hdl.handle.net/11449/74387

10.1186/1755-7682-6-2

2-s2.0-84872131878

2-s2.0-84872131878.pdf

Idioma(s)

eng

Relação

International Archives of Medicine

Direitos

openAccess

Palavras-Chave #DNA #Nanotechnology #Neoplams #Prostate #blood level #blood sampling #cancer patient #centrifugation #DNA determination #DNA extraction #human #major clinical study #male #nanotechnology #prostate cancer #reliability #spectrophotometry
Tipo

info:eu-repo/semantics/article