Validation of microbiological assay for determination of cefuroxime in injectable preparations


Autoria(s): Vieira, Daniela C.M.; Ricarte, Patrícia C.; Salgado, Hérida R.N.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

22/08/2012

Resumo

The validation of a microbiological assay, applying agar diffusion method for determination of the active of cefuroxime in power for injection, is described. Using a strain of Micrococcus luteus ATCC 9341 as the test organism, cefuroxime was measured in concentrations ranging from 30.0 to 120.0 μg/mL. The method validation showed that it is linear (r = 0.9999), precise (relative standard deviation = 0.37%) and accurate (it measured the added quantities). Microbiological assay is satisfactory for quantitation of cefuroxime in powder for injection and the validity of the proposed bioassay, which is a simple and a useful alternative methodology for cefuroxime determination in routine quality control.

Formato

746-750

Identificador

http://www.latamjpharm.org/resumenes/31/5/LAJOP_31_5_2_2.pdf

Latin American Journal of Pharmacy, v. 31, n. 5, p. 746-750, 2012.

0326-2383

http://hdl.handle.net/11449/73510

2-s2.0-84865083152

Idioma(s)

eng

Relação

Latin American Journal of Pharmacy

Direitos

closedAccess

Palavras-Chave #Cefuroxime #Cephalosporins #Microbiological assay #Quality control #Validation #cefuroxime #agar diffusion #bacterial growth #bacterial strain #bacterium culture #calibration #correlation coefficient #drug determination #drug purity #drug structure #limit of detection #limit of quantitation #microbiology #Micrococcus luteus #quality control #validation study
Tipo

info:eu-repo/semantics/article