Validation of microbiological assay for determination of cefuroxime in injectable preparations
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
27/05/2014
27/05/2014
22/08/2012
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Resumo |
The validation of a microbiological assay, applying agar diffusion method for determination of the active of cefuroxime in power for injection, is described. Using a strain of Micrococcus luteus ATCC 9341 as the test organism, cefuroxime was measured in concentrations ranging from 30.0 to 120.0 μg/mL. The method validation showed that it is linear (r = 0.9999), precise (relative standard deviation = 0.37%) and accurate (it measured the added quantities). Microbiological assay is satisfactory for quantitation of cefuroxime in powder for injection and the validity of the proposed bioassay, which is a simple and a useful alternative methodology for cefuroxime determination in routine quality control. |
Formato |
746-750 |
Identificador |
http://www.latamjpharm.org/resumenes/31/5/LAJOP_31_5_2_2.pdf Latin American Journal of Pharmacy, v. 31, n. 5, p. 746-750, 2012. 0326-2383 http://hdl.handle.net/11449/73510 2-s2.0-84865083152 |
Idioma(s) |
eng |
Relação |
Latin American Journal of Pharmacy |
Direitos |
closedAccess |
Palavras-Chave | #Cefuroxime #Cephalosporins #Microbiological assay #Quality control #Validation #cefuroxime #agar diffusion #bacterial growth #bacterial strain #bacterium culture #calibration #correlation coefficient #drug determination #drug purity #drug structure #limit of detection #limit of quantitation #microbiology #Micrococcus luteus #quality control #validation study |
Tipo |
info:eu-repo/semantics/article |