In vitro susceptibility of oral Candida albicans strains to different pH levels and calcium hydroxide saturated aqueous solution


Autoria(s): Weckwerth, Paulo Henrique; Carnietto, Cristiane; Weckwerth, Ana Carolina Villas Boas; Duarte, Marco Antonio Hungaro; Kuga, Milton Carlos; Vivan, Rodrigo Ricci
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

30/07/2012

Resumo

Candida albicans is present in the oral cavity and in the whole digestive tract of humans and other animals, being frequently related to endodontic treatment failure. The present study determined the incidence of C. albicans in the oral cavity and the susceptibility of isolates to different pH values and saturated calcium hydroxide aqueous solution at pH 12.5. Sixty-five patients attending the Endodontic Clinic at the Sagrado Coração University participated in the study. The collected samples were cultivated in selective media for C. albicans and the isolates were tested in terms of resistance to both alkaline pH and saturated aqueous solution of calcium hydroxide. In relation to time variables, yeast viability was assessed by the Sabouraud's agar culture and fluorescein diacetate and ethidium bromide fluorescent staining method. Results from the different pHs and experimental times, including those from different techniques measuring fungal viability, were compared using the chi-square and Fisher's exact tests (α=0.05). The yeasts became completely inviable after 48 h of contact with the calcium hydroxide solution. On the other hand, when exposed to the alkaline culture broth, the yeasts were found to be viable at pHs 9.5 and 10.5 for up to 7 days. In conclusion, C. albicans can only be completely inhibited by direct contact with saturated calcium hydroxide aqueous solution after 48 h of exposure.

Formato

192-198

Identificador

http://dx.doi.org/10.1590/S0103-64402012000300002

Brazilian Dental Journal, v. 23, n. 3, p. 192-198, 2012.

0103-6440

1806-4760

http://hdl.handle.net/11449/73455

10.1590/S0103-64402012000300002

S0103-64402012000300002

2-s2.0-84864226344

2-s2.0-84864226344.pdf

Idioma(s)

eng

Relação

Brazilian Dental Journal

Direitos

openAccess

Palavras-Chave #Calcium hydroxide #Candida albicans #Oral cavity #Susceptibility #biomedical and dental materials #calcium hydroxide #drug effect #human #isolation and purification #microbial sensitivity test #microbiology #mouth #pH #staining #yeast #Calcium Hydroxide #Humans #Hydrogen-Ion Concentration #Microbial Sensitivity Tests #Mouth #Root Canal Irrigants #Staining and Labeling #Yeasts
Tipo

info:eu-repo/semantics/article