Results of the GEP-ISFG collaborative study on an X-STR Decaplex

Autoria(s): Gusmão, L.; Alves, C.; Sánchez-Diz, P.; Zarrabeitia, M. T.; Abovich, M. A.; Aragón, I.; Arce, B.; Arrieta, G.; Arroyo, E.; Atmetlla, I.; Baeza, C.; Bobillo, M. C.; Cainé, L.; Campos, R.; Caraballo, L.; Carvalho, E.; Carvalho, M.; Cicarelli, Regina Maria Barretto; Comas, D.; Corach, D.; Espinoza, M.; Espinheira, M. R.; Rendo, F.; García, O.; Gomes, I.; González, A.; Hernández, A.; Hidalgo, M.; Lozano, P.; Malaghini, M.; Manzanares, D.; Martínez, B.; Martins, J. A.; Maxzud, K.; Miguel, I.; Modesti, N.; Montesino, M.; Ortiz, R.; Pestano, J. J.; Pinheiro, M. F.; Prieto, L.; Raimondi, E.; Riancho, J. A.; Rodríguez, M. B.; Salgado, I.; Salgueiro, N.; Sánchez, J. J.; Silva, S.; Toscanini, U.; Vidales, C.; Silva, C. V.; Villalobos, M. C.; Vullo, C.; Yurrebaso, I.; Zubillaga, A. I.; Carracedo, A.; Amorim, A.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)
Data(s)

27/05/2014

27/05/2014

01/08/2008
Resumo

A collaborative work was carried out by the Spanish and Portuguese ISFG Working Group with a PCR multiplex for X chromosome STRs. Markers were selected among those described as polymorphic in humans and that have been used by some laboratories in forensics. Primers and various technical methods were investigated with the aim of optimizing a multiplex for the 10 selected X-STRs. Primer mix stock solutions were sent to the laboratories that were asked to analyse two female bloodstains, taking as reference the genetic profiles from 9947A, 9948 and NA3657 samples. In this work, we report the results obtained by 30 GEP-ISFG laboratories, using this Decaplex, as well as alternative technical conditions that also produced good results. © 2008 Elsevier B.V. All rights reserved.
Formato

677-679
Identificador

http://dx.doi.org/10.1016/j.fsigss.2007.10.012

Forensic Science International: Genetics Supplement Series, v. 1, n. 1, p. 677-679, 2008.

1875-1768

http://hdl.handle.net/11449/70502

10.1016/j.fsigss.2007.10.012

2-s2.0-50649120135
Idioma(s)

eng
Relação

Forensic Science International: Genetics Supplement Series
Direitos

closedAccess
Palavras-Chave #GEP-ISFG #Multiplex PCR #X-STRs #controlled study #electrophoresis #female #forensic genetics #genetic marker #human #laboratory test #polymerase chain reaction #priority journal #reference value #short tandem repeat #silver staining #X chromosome
Tipo

info:eu-repo/semantics/article
Acesso ao item digital