Testes size in leptodactylid frogs and occurrence of multimale spawning in the genus Leptodactylus in Brazil

Although rare, some anurans show multimale spawning. Herein we present data on testes mass relative to body mass for 11 leptodactylids and report multimale spawning in Leptodactylus chaquensis and Leptodactylus podicipinus. Testes mass averaged 4.13% of body mass for L. chaquensis and 0.75% for L. podicipinus. Testes size of both leptodactylid species with multimale spawning show that they are large and close to values recorded for rhacophorid frogs with multimale breeding (0.71-7.79%). Large testes size in frogs with multimale spawning supports the sperm competition hypothesis.