Application of HPLC-NMR coupling using C30 phase in the separation and identification of flavonoids of taxonomic relevance
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
27/05/2014
27/05/2014
01/12/2000
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Resumo |
The on-line separation and identification of two important taxonomic markers for plant species of the Paepalanthus genus, the flavonoids 6-methoxykaempferol-3-O-β-D-glucopyranoside and 6-methoxykaempferol-3-O-β-D-6″(p-coumaroyl)glucopyranoside, has been performed with an HPLC-NMR coupling using C30 phase. 1D spectra have been recorded in the stopped-flow mode for the two predominant chromatographic peaks. This is the first application of HPLC-NMR coupling using C30 phase to a taxonomic problem. The technique drastically reduces the required amount of sampling for structure determination. © Springer-Verlag 2000. |
Formato |
540-542 |
Identificador |
http://dx.doi.org/10.1007/s002160000534 Fresenius' Journal of Analytical Chemistry, v. 368, n. 5, p. 540-542, 2000. 0937-0633 http://hdl.handle.net/11449/66291 10.1007/s002160000534 2-s2.0-0034332424 |
Idioma(s) |
eng |
Relação |
Fresenius' Journal of Analytical Chemistry |
Direitos |
closedAccess |
Palavras-Chave | #flavonoid #chemistry #classification #equipment design #high performance liquid chromatography #isolation and purification #medicinal plant #methodology #nuclear magnetic resonance spectroscopy #online system #standard #Chromatography, High Pressure Liquid #Equipment Design #Flavonoids #Magnetic Resonance Spectroscopy #Online Systems #Plants, Medicinal |
Tipo |
info:eu-repo/semantics/article |