Toxocariasis: Serological diagnosis by indirect antibody competition elisa


Autoria(s): Nunes, Caris Maroni; Tundisi, Regina Nardini; Heinemann, Marcos Bryan; Ogassawara, Saemi; Richtzenhain, Leonardo José
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

01/12/1999

Resumo

Toxocariasis is caused by infection of man by Toxocara canis and Toxocara, cati larvae, the common roundworm of dogs and cats. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Non specific reactions are observed mainly due to cross-reactivity with Ascaris sp antigens. This investigation aimed at developing and evaluating an indirect antibody competition ELISA (IACE) employing a specific rabbit IgG anti-Toxocara canis excretory-secretory antigens as the competition antibody. in order to improve indirect ELISA specificity performed for toxocariasis diagnosis. For that, the rabbit IgG was previously absorbed by Ascaris suum adult antigens. Sensitivity and specificity of IACE were first evaluated in 28 serum samples of mice experimentally infected with T. canis embryonated eggs. Adopting cut-off value established in this population before infection, sensitivity and specificity were 100% after 20 days post-inoculation. For human population IACE was evaluated using sera from 440 patients with clinical signs of toxocariasis and the cut-off value was established with 60 serum samples from apparently healthy individuals. Using as reference test the indirect ELISA performed by Adolfo Lutz Institute, sensitivity was 60.2%, specificity was 98% and concordance was 77.3%. Repeatability of IACE was evaluated by the inter-reactions variation coefficient (2.4%).

Formato

95-100

Identificador

http://dx.doi.org/10.1590/S0036-46651999000200006

Revista do Instituto de Medicina Tropical de Sao Paulo, v. 41, n. 2, p. 95-100, 1999.

0036-4665

http://hdl.handle.net/11449/65970

10.1590/S0036-46651999000200006

S0036-46651999000200006

2-s2.0-0033091502

Idioma(s)

eng

Relação

Revista do Instituto de Medicina Tropical de São Paulo

Direitos

openAccess

Palavras-Chave #Immunodiagnosis #Indirect antibody competition ELISA #Toxocara canis #Toxocariasis #Visceral larva migrans #helminth antibody #immunoglobulin G #parasite antigen #animal #blood #enzyme linked immunosorbent assay #female #human #immunology #methodology #mouse #sensitivity and specificity #serology #toxocariasis #Animals #Antibodies, Helminth #Antigens, Helminth #Enzyme-Linked Immunosorbent Assay #Female #Humans #Immunoglobulin G #Mice #Sensitivity and Specificity #Serologic Tests
Tipo

info:eu-repo/semantics/article