Paracoccidioides brasiliensis-gp43 used as paracoccidioidin


Autoria(s): Saraiva, E. C O; Altemani, A.; Franco, M. F.; Unterkircher, C. S.; Camargo, Z. P.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

01/12/1996

Resumo

A purified glycoprotein of 43 000 daltons from Paracoccidioides brasiliensis (gp43) was tested as paracoccidioidin in delayed-type hypersensitivity (DTH) tests in both experimental animals (guinea pig and mice) and patients with paracoccidioidomycosis (PCM). The gp43 paracoccidioidin was compared with the traditional Fava Netto antigen (AgFN). In guinea pigs, the intradermal injection of 2 μg of gp43 showed a similar response to those obtained with AgFN, showing in histological sections a population of lymphoid cells that participate in DTH. In mice, gp43 at a dose of 3.75μg showed positive DTH response. The use of gp43 as paracoccidioidin in humans showed that this molecule can be used to evaluate the DTH response in patients with PCM. Of 25 PCM patients studied, 48% were positive to gp43 while only 28% were positive to AgFN; 12 PCM patients were completely anergic to both antigens. Considering only those 13 PCM patients who were responsive to gp43 and/or to AgFN, 92.3% reacted against gp43 and 53.8% reacted against AgFN (P < 0.05). Gp43 skin test responses (13.67 ± 9.56 mm) were significantly larger than those obtained with AgFN (8.43 ±3.69 mm). Immunohistochemical study of the human skin showed a perivascular inflammatory response constituted predominantly by T lymphocytes, macrophages and polymorphonuclear leukocytes. © 1996 ISHAM.

Formato

155-161

Identificador

http://dx.doi.org/10.1080/02681219680000261

Journal of Medical and Veterinary Mycology, v. 34, n. 3, p. 155-161, 1996.

0268-1218

http://hdl.handle.net/11449/64904

10.1080/02681219680000261

WOS:A1996UN54900001

2-s2.0-0029661850

Idioma(s)

eng

Relação

Journal of Medical and Veterinary Mycology

Direitos

closedAccess

Palavras-Chave #Gp43 #P. Brasiliensis #Paracoccidioidin #Paracoccidioidomycosis #fungus antigen #fungal protein #paracoccidioidin #animal experiment #controlled study #delayed hypersensitivity #guinea pig #human #human tissue #immune response #immunohistochemistry #inflammation #mouse #nonhuman #paracoccidioides brasiliensis #serum #skin #south american blastomycosis #adult #animal #chemically induced disorder #comparative study #immunology #male #microbiology #Paracoccidioides #skin test #South American blastomycosis #Adult #Animals #Fungal Proteins #Guinea Pigs #Humans #Hypersensitivity, Delayed #Male #Mice #Skin Tests
Tipo

info:eu-repo/semantics/article