Sex determination of murine and bovine embryos using cytotoxicity and immunofluorescence assays


Autoria(s): Hossepian de Lima, V. F M; Moreira-Filho, C. A.; De Bem, A. R.; Jorge, W.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

01/06/1993

Resumo

Murine and bovine embryos were sexed by using polyclonal and monoclonal anti-H-Y antibodies in cytotoxic and immunofluorescence assays. The specificity of 3 different sources of antibodies are compared. Sexing was accomplished by complement-mediated cytotoxicity or by H-Y antigen detection using a anti-mouse Ig fluorescein conjugate. Cytogenetic ascertainment for correct sex identification yielded a score of about 85% in both species, while the immunofluorescence assay yielded a score of 83%. © 1993.

Formato

1343-1352

Identificador

http://dx.doi.org/10.1016/0093-691X(93)90236-X

Theriogenology, v. 39, n. 6, p. 1343-1352, 1993.

0093-691X

http://hdl.handle.net/11449/64377

10.1016/0093-691X(93)90236-X

WOS:A1993LF25800013

2-s2.0-38249001637

Idioma(s)

eng

Relação

Theriogenology

Direitos

closedAccess

Palavras-Chave #anti-H-Y antibodies #embryo #immunological assays #sexing
Tipo

info:eu-repo/semantics/article