Sulfonation and anticoagulant activity of botryosphaeran from Botryosphaeria rhodina MAMB-05 grown on fructose
| Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
|---|---|
| Data(s) |
20/05/2014
20/05/2014
01/10/2009
|
| Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Processo FAPESP: 05/53879-3 Botryosphaeran (EPS(FRU)), an exopolysaccharide of the beta-(1 -> 3,1 -> 6)-D-glucan type with 31% branching at C-6, is produced by the fungus Botryosphaeria rhodina MAMB-05 when grown on fructose as carbon source. Botryosphaeran was derivatized by sulfonation to induce anticoagulant activity. The effectiveness of the sulfonation reaction by chlorosulfonic acid in pyridine was monitored by the degree of substitution and FT-IR analysis of the sulfonated EPS(FRU) (once sulfonated, EPS(FRU SULF); and re-sulfonated, EPS(FRU RESULF)). Activated partial thromboplastin time (APTT) and thrombin time (TT) tests of EPS(FRU RESULF) indicated significant in vitro anticoagulant activity that was dose-dependent. EPS(FRU) did not inhibit any of the coagulation tests. (C) 2009 Elsevier B.V. All rights reserved. |
| Formato |
305-309 |
| Identificador |
http://dx.doi.org/10.1016/j.ijbiomac.2009.06.004 International Journal of Biological Macromolecules. Amsterdam: Elsevier B.V., v. 45, n. 3, p. 305-309, 2009. 0141-8130 http://hdl.handle.net/11449/41760 10.1016/j.ijbiomac.2009.06.004 WOS:000269393500017 |
| Idioma(s) |
eng |
| Publicador |
Elsevier B.V. |
| Relação |
International Journal of Biological Macromolecules |
| Direitos |
closedAccess |
| Palavras-Chave | #Botryosphaeria rhodina MAMB-05 #Exopolysaccharide #beta-(1 -> 3,1 -> 6)-D-Glucan #Sulfonation #Anticoagulant activity |
| Tipo |
info:eu-repo/semantics/article |
