Potential role for dog fleas in the cycle of Leishmania spp.


Autoria(s): Prado Albuquerque Ferreira, Marilia Gabriele; Fattori, Karina Reinaldo; Souza, Fausto; Felix Lima, Valeria Marcal
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

28/10/2009

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Several species of Leishmania spp. cause diseases in humans that range from self-healing cutaneous lesions to fatal visceral leishmaniosis. it has been observed that besides being transmitted by sand flies, Leishmania spp. may also be transmitted by arthropods such as ticks and fleas. To investigate the possible role of dog fleas in the transmission of Leishmania spp., Ctenocefalides felis were removed from 22 dogs which were positive according to ELISA and rK-39 tests. A C. felis sample from each of the 22 dogs was used to infect a hamster. The 22 hamsters were euthanized 4 months after infection with the fleas and the blood was subjected to ELISA to detect antibody anti-Leishmania spp., and the spleen samples were submitted to PCR for detection of Leishmania spp. DNA. PCR and ELISA were both positive in 18.1% (4/22), with PCR alone being positive in 45% (10/22) and ELISA alone in only 9% (2/22). These results suggest the participation of dog fleas in the Leishmania spp. cycle. Confirmation that C. felis indeed transmit leishmaniosis to dogs requires new strategies against leishmaniosis to be enforced by public health authorities and which focus on better ways to keep dogs free of fleas. (C) 2009 Elsevier B.V. All rights reserved.

Formato

150-154

Identificador

http://dx.doi.org/10.1016/j.vetpar.2009.06.026

Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 165, n. 1-2, p. 150-154, 2009.

0304-4017

http://hdl.handle.net/11449/41297

10.1016/j.vetpar.2009.06.026

WOS:000271160200023

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Veterinary Parasitology

Direitos

closedAccess

Palavras-Chave #Canine visceral leishmaniosis #Fleas #Leishmania spp. #PCR #ELISA
Tipo

info:eu-repo/semantics/article