Wolbachia in Anastrepha Fruit Flies (Diptera: Tephritidae)


Autoria(s): Coscrato, Virginia E.; Braz, Antonio S. K.; Perondini, Andre L. P.; Selivon, Denise; Marino, Celso L.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/09/2009

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Processo FAPESP: 03/02693-4

Processo FAPESP: 03/00069-9

Endosymbiotic bacteria of the genus Wolbachia are widespread among arthropods and cause a variety of reproductive abnormalities, such as cytoplasmic incompatibility, thelytokous parthenogenesis, male-killing, and host feminization. In this study, we used three sets of Wolbachia-specific primers (16S rDNA, ftsZ, and wsp) in conjunction with the polymerase chain reaction (PCR), cloning and sequencing to study the infection of fruit flies (Anastrepha spp. and Ceratitis capitata) by Wolbachia. The flies were collected at several localities in Brazil and at Guayaquil, Ecuador. All of the fruit flies studied were infected with Wolbachia supergroup A, in agreement with the high prevalence of this group in South America. Phylogenetic analysis showed that the wsp gene was the most sensitive gene for studying the relationships among Wolbachia strains. The Wolbachia sequences detected in these fruit flies were similar to those such as wMel reported for other fruit flies. These results show that the infection of Anastrepha fruit flies by Wolbachia is much more widespread than previously thought.

Formato

295-301

Identificador

http://dx.doi.org/10.1007/s00284-009-9433-8

Current Microbiology. New York: Springer, v. 59, n. 3, p. 295-301, 2009.

0343-8651

http://hdl.handle.net/11449/41240

10.1007/s00284-009-9433-8

WOS:000268775800013

Idioma(s)

eng

Publicador

Springer

Relação

Current Microbiology

Direitos

closedAccess

Tipo

info:eu-repo/semantics/article