A Preliminary and Qualitative Metallomics Study of Mercury in the Muscle of Fish from Amazonas, Brazil


Autoria(s): Moraes, Paula M.; Santos, Felipe A.; Padilha, Cilene C. F.; Vieira, Jose C. S.; Zara, Luiz F.; Padilha, Pedro de M.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/12/2012

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Processo FAPESP: 10/51332-5

Processo FAPESP: 09/54856-8

This paper presents preliminary findings for a metallomics study of mercury in the muscle of the fish species from Amazonas, Brazil, after protein separation by two-dimensional polyacrylamide gel electrophoresis and subsequent evaluation of mercury by synchrotron radiation X-ray fluorescence. The fluorescence spectra revealed mercury in two protein spots. The mercury-containing protein spots showed molecular weights of 20.8 +/- 0.7 and 19.8 +/- 0.5 kDa and isoelectric points of 5.6 +/- 0.2 and 7.5 +/- 0.3, respectively.

Formato

195-199

Identificador

http://dx.doi.org/10.1007/s12011-012-9502-x

Biological Trace Element Research. Totowa: Humana Press Inc, v. 150, n. 1-3, p. 195-199, 2012.

0163-4984

http://hdl.handle.net/11449/40765

10.1007/s12011-012-9502-x

WOS:000312075800028

Idioma(s)

eng

Publicador

Humana Press Inc

Relação

Biological Trace Element Research

Direitos

closedAccess

Palavras-Chave #Metallomics study #Mercury in fish #2D PAGE #SR XRF
Tipo

info:eu-repo/semantics/article