Free activity in the cage associated with body weight and restoration of bone structural and mechanical properties in growing rats after hindlimb unloading


Autoria(s): Barbosa, Adelton Andrade; Del Carlo, Ricardo Junqueira; Galvao, Simone Rezende; Louzada, Mário Jefferson Quirino; Caldoncelli Franco, Frederico Souzalima; Fernandes, Barbara Braga; Carneiro-Junior, Miguel Araujo; Soares Ramos, Regiane Maria; Drummond, Lucas Rios; Natali, Antonio Jose
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/07/2012

Resumo

We investigated the importance of daily free activity in the cage and body weight gain during the recovering of bone structural and mechanical properties in growing rats after hindlimb unloading. Eight-week-old male Wistar rats were randomly divided into control (CG, n=24) and suspended (SG, n=24) groups. Animals from SG underwent a four-week hindlimb unloading period by tail-suspension. Animals from CG and those from SG after release were kept in collective cages and sacrificed at the age of 12, 16 and 20 weeks. Both femurs were removed and its area, bone mineral density (BMD), resistance to failure and stiffness were determined. Four-week hindlimb unloading decreased (p < 0.05) body weight (CG, 373.00 +/- 9.47 vs. SG, 295.86 +/- 9.19 g), BMD (CG, 0.19 +/- 0.01 vs. SG, 0.15 +/- 0.01 g/cm(2)), bone resistance to failure (CG, 147.75 +/- 5.05 vs. SG, 96.40 +/- 5.95 N) and stiffness (CG, 0.38 +/- 0.01 vs. SG, 0.23 +/- 0.02 N/m). Eight weeks of free activity in cage recovered (p > 0.05) the body weight (CG, 472.75 +/- 14.11 vs. SG, 444.75 +/- 18.91 g), BMD (CG, 0.24 +/- 0.01 vs. SG, 0.22 +/- 0.01 g/cm(2)), bone resistance to failure (CG, 195.73 +/- 10.06 vs. SG, 178.45 +/- 8.48 N) and stiffness (CG, 0.56 +/- 0.02 vs. SG, 0.47 +/- 0.03 N/m) of SG animals. Body weight correlated strongly with bone structural and mechanical properties (p < 0.0001). In conclusion, free activity in the cage associated with body weight gain restored bone structural and mechanical properties in growing rats after hindlimb unloading.

Formato

660-666

Identificador

http://www.seer.ufu.br/index.php/biosciencejournal/article/view/9159

Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 28, n. 4, p. 660-666, 2012.

1516-3725

http://hdl.handle.net/11449/40069

WOS:000308677400019

WOS000308677400019.pdf

Idioma(s)

eng

Publicador

Universidade Federal de Uberlândia (UFU)

Relação

Bioscience Journal

Direitos

openAccess

Palavras-Chave #Osteopenia #Bone mineral density #Bone stiffness
Tipo

info:eu-repo/semantics/article