Progesterone upregulates GATA-1 on erythroid progenitors cells in liquid culture
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/09/2002
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Resumo |
Steroids hormones modify the hematological features of homozygous sickle cell disease, including the levels of fetal hemoglobin. We used semi-quantitative RT-PCR analysis of GATA-1, GATA-2, NF-E2, and gamma-globin mRNA levels in a two-phase liquid culture system of human adult erythroid cells in order to assay the effect of progesterone upon gene expression. The levels of expression of GATA-1 and gamma-globin mRNA were significantly increased in cells treated with progesterone compared to untreated cells (1.7- to 2.0-fold). Progesterone treatment did not produce any stimulatory effect upon GATA-2 and NF-E2 mRNA expression. Differences in the synthesis of HbF protein could not be detected by flow cytometry, although we observed a small difference in mean intensity fluorescence between cells treated and cells untreated with progesterone on days 7 and 9. Using anti-transferrin receptor and anti-glycophorin A antibodies, we verified that addition of progesterone did not cause any change in erythroid proliferation and differentiation. In conclusion, it is possible that the increased expression of gamma-globin mRNA after progesterone treatment observed in this study may be related to the increased GATA-1 mRNA expression. Interactions of the steroid receptors with the basal transcriptional machinery and with transcription factors might mediate their transcriptional effects. (C) 2002 Elsevier B.V. (USA). |
Formato |
213-224 |
Identificador |
http://dx.doi.org/10.1006/bcmd.2002.0551 Blood Cells Molecules and Diseases. San Diego: Academic Press Inc. Elsevier B.V., v. 29, n. 2, p. 213-224, 2002. 1079-9796 http://hdl.handle.net/11449/40040 10.1006/bcmd.2002.0551 WOS:000179991100009 |
Idioma(s) |
eng |
Publicador |
Elsevier B.V. |
Relação |
Blood Cells Molecules and Diseases |
Direitos |
closedAccess |
Palavras-Chave | #HbF #progesterone #transcription factors #RT-PCR #gene expression |
Tipo |
info:eu-repo/semantics/article |