Exploiting Cascade Reactions in Bienzyme Layer-by-Layer Films
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
06/10/2011
|
Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Control of supramolecular architectures inherent in layer-by-layer (LbL) films allows far synergistic effects be, tween the film components, as in reactions under controlled conditions. In this paper we show that a bienzyme system can be produced in LbL films, in which the product of a reaction catalyzed by invertase (INV) is used in a catalysis process involving glucose oxidase (GOx). As a proof of principle, simultaneous detection of glucose and sucrose is demonstrated for an optimized film architecture comprising poly allyllamine hydrochloride (PAH) alternated with INV + GOx layers (PAH/(GOx + INV))(10). Though the sensitivity for sucrose is low because the product from the reaction with invertase has low electroactivity, the results confirm that cascade reactions can be performed in a controlled manner in LbL films with possible applications in sensors and bioelectrochemical devices. |
Formato |
19136-19140 |
Identificador |
http://dx.doi.org/10.1021/jp207610w Journal of Physical Chemistry C. Washington: Amer Chemical Soc, v. 115, n. 39, p. 19136-19140, 2011. 1932-7447 http://hdl.handle.net/11449/39891 10.1021/jp207610w WOS:000295245500029 |
Idioma(s) |
eng |
Publicador |
Amer Chemical Soc |
Relação |
Journal of Physical Chemistry C |
Direitos |
closedAccess |
Tipo |
info:eu-repo/semantics/article |