Crystallization and preliminary X-ray diffraction analysis of an anti-H(O) lectin from Lotus tetragonolobus seeds
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/07/2006
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Resumo |
The seed lectin from Lotus tetragonolobus (LTA) has been crystallized. The best crystals grew over several days and were obtained using the vapour-diffusion method at a constant temperature of 293 K. A complete structural data set was collected at 2.00 angstrom resolution using a synchrotron-radiation source. LTA crystals were found to be monoclinic, belonging to space group P2(1), with unit-cell parameters a = 68.89, b = 65.83, c = 102.53 angstrom, alpha = gamma = 90, beta = 92 degrees. Molecular replacement yielded a solution with a correlation coefficient and R factor of 34.4 and 51.6%, respectively. Preliminary analysis of the molecular-replacement solution indicates a new quaternary association in the LTA structure. Crystallographic refinement is under way. |
Formato |
680-683 |
Identificador |
http://dx.doi.org/10.1107/S1744309106021312 Acta Crystallographica Section F-structural Biology and Crystallization Communications. Oxford: Blackwell Publishing, v. 62, p. 680-683, 2006. 1744-3091 http://hdl.handle.net/11449/36205 10.1107/S1744309106021312 WOS:000238656200020 |
Idioma(s) |
eng |
Publicador |
Blackwell Publishing |
Relação |
Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
Direitos |
closedAccess |
Tipo |
info:eu-repo/semantics/article |