Determination of the betaine content of feed ingredients using high-performance liquid chromatography


Autoria(s): Chendrimada, T. P.; Neto, M. G.; Pesti, G. M.; Davis, A. J.; Bakalli, R. I.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/10/2002

Resumo

A series of studies was conducted to establish a methodology for the accurate and efficient determination of betaine in different feed ingredients. The final methodology involves an extraction step in which the feed sample is heated for 3h in a methanolic KOH solution using a Goldfisch apparatus. Impurities are removed by the addition of activated charcoal and concentrated (36%) HCl. After centrifugation the extractant is passed through a strong cation exchange resin (Dowex 50W-X12, H+). The betaine retained in the column is eluted with 1.5 N HCl. A 2 nil aliquot of the elute is air dried and reconstituted with 1 ml of deionised water. HPLC separation with a cation exchange column (Partisil SCX-10) is used for the separation of betaine from other compounds. The mobile phase is kept constant at 50mm KH2PO4 in water, and eluted compounds are detected by UV absorbance (200nm). The flow rate is maintained at 1.5ml min(-1). This assay is very accurate over the range of betaine concentrations from 15 to 650 mug ml(-1), with a lower detection limit in feeds of approximately 500 mug g(-1) when 4g of sample is extracted. Recovery assays done with standard betaine hydrochloride and hard red wheat resulted in a consistent recovery of 80%. Betaine content was quantified in several feed ingredients, including alfalfa (1.77 mg kg(-1)), wheat (3.96 mg kg(-1)), wheat middlings (4.98 mg kg(-1)) and poultry meal (0.77 mg kg(-1)). Betaine in corn and soybean meal was not detectable by this method, even when 16g of sample was used (<125 mg kg(-1)). Betaine present in several feed ingredients should influence choline supplementation to animal feeds and may have implications for human health. (C) 2002 Society of Chemical Industry.

Formato

1556-1563

Identificador

http://dx.doi.org/10.1002/jsfa.1214

Journal of the Science of Food and Agriculture. W Sussex: John Wiley & Sons Ltd, v. 82, n. 13, p. 1556-1563, 2002.

0022-5142

http://hdl.handle.net/11449/34786

10.1002/jsfa.1214

WOS:000178354300021

Idioma(s)

eng

Publicador

Wiley-Blackwell

Relação

Journal of the Science of Food and Agriculture

Direitos

closedAccess

Palavras-Chave #betaine #feed #HPLC
Tipo

info:eu-repo/semantics/article