X chromosome heterochromatic pattern and nucleolar synthesis in pupal ovaries of Aedes aegypti


Autoria(s): de Sousa, R. D.; Bicudo, HEMD
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/01/1998

Resumo

C-banding and silver-staining techniques were used to examine pupal ovaries of Aedes aegypti from Sao Jose do Rio Preto (Brazil). Silver staining in ovary cystocytes showed two basic patterns relative to the nucleolar morphology: viz (1) a single, compact small body; and (2) multiple bodies encompassing large nuclear areas. These two types of cystocytes were present in the ratio of 7:1, which is the same as the number of nurse cells and oocytes, respectively, in each follicle. This suggests the possibility of eventually using such a nucleolar morphological difference to recognize both cell types in developmental stages before emergence. Silver nitrate staining in metaphase chromosomes revealed centromeric bands on all six chromosomes. The C-banding pattern in metaphase chromosomes showed an intercalary band in one of the X arms, as described previously in other populations. In ovary cystocytes (pachytene stage) this C-positive band seemed to consist of two chromomeres. Phase contrast microscopy showed that the nucleolus was associated with the distal chromomere of this intercalary C-band, indicating that the nucleolus organizer region was located in that part of the heterochromatic band.

Formato

185-193

Identificador

http://cat.inist.fr/?aModele=afficheN&cpsidt=10566824

Cytobios. Cambridge: Faculty Press, v. 93, n. 374, p. 185-193, 1998.

0011-4529

http://hdl.handle.net/11449/34238

WOS:000076048100006

Idioma(s)

eng

Publicador

Faculty Press

Relação

Cytobios

Direitos

closedAccess

Palavras-Chave #Aedes aegypti #nucleolus #nucleolar organizer regions #heterochromatic pattern
Tipo

info:eu-repo/semantics/article