Predisposing genes and increased chromosome aberrations in lung cancer cigarette smokers


Autoria(s): ConfortiFroes, N.; ElZein, R.; AbdelRahman, S. Z.; Zwischenberger, J. B.; Au, W. W.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

05/09/1997

Resumo

Genotoxic effects linking cigarette smoking with lung cancer have not been consistently demonstrated, therefore claims for the cause-effect relationships are vigorously contested. Using matched populations of 22 lung cancer patients who have been cigarette smokers (LCP), 22 non-cancerous cigarette smokers (SC) and 13 non-smokers (NSC), we have applied the fluorescence in situ hybridization (FISH) tandem probe assay to elucidate the frequency of chromosome breakage among the participants. Two probes were used, a classical satellite probe which hybridizes to the large heterochromatin region of chromosome 1, and an alpha-satellite probe which targets a small region adjacent to the heterochromatin probe. The highest frequency of structural aberrations was observed in LCP (1.4 +/- 0.1) followed by SC (1.25 +/- 0.1) and NSC (0.4 +/- 0.1). Aberration frequencies were not significantly different between LCP and SC (p > 0.05), however, a statistically significant difference was detected between the smoker populations combined (LCP and SC) and the NSC (p < 0.001). The breakage frequencies showed a positive correlation with duration of smoking for LCP (r = 0.5; p < 0.01), but not for SC (P > 0.05). In addition, the aberration frequencies were influenced by the inheritance of polymorphic glutathione S-transferase (GST) genes. LCPs missing one or the other GST (GSTM1 or GSTT1) genes were found to have significantly higher chromosome breaks compared to LCPs with both genes present (p < 0.05), Our data indicate that genetic predisposition and chromosome aberrations may be mechanistically related to the initiation of lung carcinogenesis; therefore, they may be useful biomarkers for lung cancer among cigarette smokers. (C) 1997 Elsevier B.V. B.V.

Formato

53-59

Identificador

http://dx.doi.org/10.1016/S0027-5107(97)00106-1

Mutation Research-fundamental and Molecular Mechanisms of Mutagenesis. Amsterdam: Elsevier B.V., v. 379, n. 1, p. 53-59, 1997.

0027-5107

http://hdl.handle.net/11449/33227

10.1016/S0027-5107(97)00106-1

WOS:A1997YC14500006

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Mutation Research: Fundamental and Molecular Mechanisms of Mutagenesis

Direitos

closedAccess

Palavras-Chave #chromosome aberration #polymorphic GST gene #lung cancer #cigarette smoker
Tipo

info:eu-repo/semantics/article