Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia?


Autoria(s): Carraretto, Antônio Roberto; Vianna Filho, Pedro Thadeu Galvão; Castiglia, Yara Marcondes Machado; Golim, Márjorie de Assis; Souza, Aparecida Vitória Gonçalves de; Carvalho, Lídia Raquel de; Deffune, Elenice; Vianna, Pedro Thadeu Galvão
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/03/2013

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

PURPOSE: To study the effect of isoflurane (Iso) or propofol (Prop) anesthesia on renal ischemia/reperfusion injury (IRI) during transient hyperglycemia. METHODS: Thirty six rats were randomly assigned into six groups of six animals each: PHS (Sham-Prop=1mg.kg-1.min-1 + Hyperglycemia=2.5g.kg-1 of glucose solution administered intraperitoneally); HIS (Sham-Iso + Hyperglycemia); PHI (Prop + Hyperglycemia + Ischemia); IHI (Iso + Hyperglycemia + Ischemia); PI (Prop + Ischemia), and II (Iso + Ischemia). After 30 minutes of anesthesia induction, right nephrectomy was performed (all animals) and the left renal artery was clamped during 25 minutes (ischemia). The animals were sacrificed after 24 hours and blood collection (to dose creatinine) and left kidney removal were performed for histological analysis, and flow cytometry (FCM): percentage of initial apoptosis (APTi) and viable cells (VC). RESULTS: Serum creatinine (mg/dL) was statistically different in groups PHI (3.60±0.40) and IHI (3.23±1.08), p<0.05. Histological analysis was statistically different in groups PHI (4.0[4.0;5.0]) and IHI (4.5[4.0;5.0]), p<0.05. APTi percentage was statistically different in groups PHI (73.2±7.1), and IHI (48.1±14). VC percentage was statistically different in groups PHI (25.8±6.9) and IHI (38.5±9.2), p<0.05. CONCLUSIONS: Propofol and isoflurane showed the same level of protection against ischemia/reperfusion injury in the normoglycemic groups. Transient hyperglycemia is associated with an increase in IRI.

Formato

161-166

Identificador

http://dx.doi.org/10.1590/S0102-86502013000300001

Acta Cirúrgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 3, p. 161-166, 2013.

0102-8650

http://hdl.handle.net/11449/29019

10.1590/S0102-86502013000300001

S0102-86502013000300001

WOS:000316126000001

S0102-86502013000300001.pdf

Idioma(s)

eng

Publicador

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia

Relação

Acta Cirúrgica Brasileira

Direitos

openAccess

Palavras-Chave #Kidney #Ischemia #Reperfusion #Hyperglycemia #Propofol #Isoflurane #Rats
Tipo

info:eu-repo/semantics/article