Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/01/2008
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Resumo |
Fractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL(-1) and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1: 1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system. |
Formato |
782-787 |
Identificador |
http://dx.doi.org/10.1590/S0103-50532008000400024 Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 19, n. 4, p. 782-787, 2008. 0103-5053 http://hdl.handle.net/11449/25839 10.1590/S0103-50532008000400024 S0103-50532008000400024 WOS:000256957100023 |
Idioma(s) |
eng |
Publicador |
Soc Brasileira Quimica |
Relação |
Journal of the Brazilian Chemical Society |
Direitos |
openAccess |
Palavras-Chave | #biosensor #hepatitis C virus #fractional factorial design |
Tipo |
info:eu-repo/semantics/article |