Regulation of xylanase in Aspergillus phoenicis: a physiological and molecular approach
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/04/2008
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Resumo |
Microbial xylanolytic enzymes have a promising biotechnological potential, and are extensively applied in industries. In this study, induction of xylanolytic activity was examined in Aspergillus phoenicis. Xylanase activity induced by xylan, xylose or beta-methylxyloside was predominantly extracellular (93-97%). Addition of 1% glucose to media supplemented with xylan or xylose repressed xylanase production. Glucose repression was alleviated by addition of cAMP or dibutyryl-cAMP. These physiological observations were supported by a Northern analysis using part of the xylanase gene ApXLN as a probe. Gene transcription was shown to be induced by xylan, xylose, and beta-methylxyloside, and was repressed by the addition of 1% glucose. Glucose repression was partially relieved by addition of cAMP or dibutyryl cAMP. |
Formato |
237-244 |
Identificador |
http://dx.doi.org/10.1007/s10295-007-0290-9 Journal of Industrial Microbiology & Biotechnology. Heidelberg: Springer Heidelberg, v. 35, n. 4, p. 237-244, 2008. 1367-5435 http://hdl.handle.net/11449/25265 10.1007/s10295-007-0290-9 WOS:000254249600005 |
Idioma(s) |
eng |
Publicador |
Springer Heidelberg |
Relação |
Journal of Industrial Microbiology & Biotechnology |
Direitos |
closedAccess |
Palavras-Chave | #fungi #Aspergillus phoenicis #xylanase #cAMP #gene transcription |
Tipo |
info:eu-repo/semantics/article |