Comparison of the photodynamic fungicidal efficacy of methylene blue, toluidine blue, malachite green and low-power laser irradiation alone against Candida albicans


Autoria(s): Souza, Rodrigo C.; Junqueira, Juliana Campos; Rossoni, Rodnei D.; Pereira, Cristiane A.; Munin, Egberto; Jorge, Antonio O. C.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/05/2010

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Processo FAPESP: 06/54896-1

This study was to evaluate specific effects of photodynamic therapy (energy density 15.8 J/cm(2), 26.3 J/cm(2) and 39.5 J/cm(2)) using methylene blue, toluidine blue and malachite green as photosensitizers and low-power laser irradiation on the viability of Candida albicans. Suspensions of C. albicans containing 10(6) cells/ml were standardized in a spectrophotometer. For each dye, 120 assays, divided into four groups according to the following experimental conditions, were carried out: laser irradiation in the presence of the photosensitizer; laser irradiation only; treatment with the photosensitizer only; no exposure to laser light or photosensitizer. Next, serial dilutions were prepared and seeded onto Sabouraud dextrose agar for the determination of the number of colony-forming units per milliliter (CFU/ml). The results were subjected to analysis of variance and the Tukey test (P < 0.05). Photodynamic therapy using the photosensitizers tested was effective in reducing the number of C. albicans.. The number of CFU/ml was reduced by between 0.54 log(10) and 3.07 log(10) and depended on the laser energy density used. Toluidine blue, methylene blue and malachite green were effective photosensitizers in antimicrobial photodynamic therapy against C. albicans, as was low-power laser irradiation alone.

Formato

385-389

Identificador

http://dx.doi.org/10.1007/s10103-009-0706-z

Lasers In Medical Science. Artington: Springer London Ltd, v. 25, n. 3, p. 385-389, 2010.

0268-8921

http://hdl.handle.net/11449/22641

10.1007/s10103-009-0706-z

WOS:000275444500011

Idioma(s)

eng

Publicador

Springer London Ltd

Relação

Lasers in Medical Science

Direitos

closedAccess

Palavras-Chave #Photodynamic therapy #Low-power laser irradiation #Candida albicans #Toluidine blue #Methylene blue #Malachite green
Tipo

info:eu-repo/semantics/conferencePaper