Snake Venomic of Crotalus durissus terrificus-Correlation with Pharmacological Activities


Autoria(s): Georgieva, Dessislava; Oehler, Michaela; Seifert, Jana; von Bergen, Martin; Arni, Raghuvir K.; Genov, Nicolay; Betzel, Christian
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/05/2010

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Processo FAPESP: 07/54865-1

The snake venomic of Crotalus durissus terrificus was analyzed by 2-D and 1-D electrophoresis and subsequent MS/MS and enzymatic assays. The venomic of the South American rattlesnake comprises toxins from seven protein families: phospholipases A(2), serine proteinases, ecto-5'-nucleotidases, metalloproteinases, nerve growth factors, phosphodiesterases, and glutaminyl cyclase. The venom toxin composition correlates with the clinical manifestation of the crotalinae snake bites and explains pathological effects of the venom such as neurotoxicity, systemic myonecrosis, hemostatic disorders, myoglobinuria, and acute renal failure. The vast majority of toxins are potentially involved in neurotoxicity, myotoxicity, and coagulopathy. The predominant venom components are neurotoxic phospholipases A2 and serine proteinases. The venom is a rich source of 5'-nucleotidases (7.8% of the identified toxins) inducing hemostatic disorders. Analysis of the venom protein composition provided a catalogue for secreted toxins. The venomic composition of Crotalus d. terrificus and venom gland transcriptome of the synonymous subspecies Crotalus d. collilineatus show differences in the occurrence of protein families and in the abundance of toxins. Some of the venom components identified by the proteomic analysis were not reported in the transcriptome of the Crotalus d. collilineatus venom gland. Enzymatic activities of the Crotalus d. terrificus venom were determined and correlated with the proteomic composition.

Formato

2302-2316

Identificador

http://dx.doi.org/10.1021/pr901042p

Journal of Proteome Research. Washington: Amer Chemical Soc, v. 9, n. 5, p. 2302-2316, 2010.

1535-3893

http://hdl.handle.net/11449/22055

10.1021/pr901042p

WOS:000277353200022

Idioma(s)

eng

Publicador

Amer Chemical Soc

Relação

Journal of Proteome Research

Direitos

closedAccess

Palavras-Chave #Snake venomic #Crotalus durissus terrificus #2-D electrophoresis #electrospray mass spectrometry
Tipo

info:eu-repo/semantics/article