Amino acid sequence of a myotoxic Lys49-phospholipase A(2) homologue from the venom of Cerrophidion (Bothrops) godmani


Autoria(s): de Sousa, M. V.; Morhy, L.; Arni, R. K.; Ward, R. J.; Diaz, C.; Gutierrez, J. M.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

19/05/1998

Resumo

The complete amino acid sequence of myotoxin II (godMT-II), a myotoxic phospholipase A( 2 )(PLA(2)) homologue from the venom of the Central American crotaline snake Cerrophidion (Bothrops) godmani, was determined by direct protein sequencing methods. GodMT-II is a class II PLA, showing a Lys instead of Asp at position 49. An additional substitution in the calcium binding loop region (Asn instead of Tyr at position 28) suggests the lack of enzymatic activity observed in this toxin is due to loss of its ability to bind the co-factor Ca2+, since the residues involved in forming the catalytic network of PLA(2)s (His-48, Tyr-52 and Asp-99) an conserved in godMT-II. This myotoxin shows highest sequence homology with other Lys-49 PLA(2)s from Bothrops, Agkistrodon and Trimeresurus species, suggesting that they constitute a conserved family of proteins, yet in contrast presents lower homology with Bothrops asper myotoxin III, a catalytically-active PLA(2). The C-terminal region of godMT-II, which is rich in cationic and hydrophobic residues, shares high sequence homology to the corresponding region in the myotoxin II from B. asper, which has been proposed to play an important role in the Ca2+-independent membrane damaging activity. (C) 1998 Elsevier B.V. B.V. All rights reserved.

Formato

204-208

Identificador

http://dx.doi.org/10.1016/S0167-4838(98)00023-5

Biochimica Et Biophysica Acta-protein Structure and Molecular Enzymology. Amsterdam: Elsevier B.V., v. 1384, n. 2, p. 204-208, 1998.

0167-4838

http://hdl.handle.net/11449/21971

10.1016/S0167-4838(98)00023-5

WOS:000074292100003

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Biochimica Et Biophysica Acta-protein Structure and Molecular Enzymology

Direitos

closedAccess

Palavras-Chave #amino acid #myotoxic #venom
Tipo

info:eu-repo/semantics/article