Pectinase production by a Brazilian thermophilic fungus Thermomucor indicae-seudaticae N31 in solid-state and submerged fermentation
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/06/2010
|
Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Thermophilic organisms produce thermostable enzymes, which have a number of applications, justifying the interest in the isolation of new thermophilic strains and study of their enzymes. Thirty-four thermophilic and thermotolerant fungal strains were isolated from soil, organic compost, and an industrial waste pile based on their ability to grow at 45A degrees C and in a liquid medium containing pectin as the only carbon source. Among these fungi, 50% were identified at the genus level as Thermomyces, Aspergillus, Monascus, Chaetomium, Neosartoria, Scopulariopsis, and Thermomucor. All isolated strains produced pectinase during solid-state fermentation (SSF). The highest polygalacturonase (PG) activity was obtained in the culture medium of thermophilic strain N31 identified as Thermomucor indicae-seudaticae. Under SSF conditions on media containing a mixture of wheat bran and orange bagasse (1 : 1) at 70% of initial moisture, this fungus produced the maximum of 120 U/ml of exo-PG, while in submerged fermentation (SmF) it produced 13.6 U/ml. The crude PG from SmF was more thermostable than that from SSF and exhibited higher stability in acidic pH. |
Formato |
306-313 |
Identificador |
http://dx.doi.org/10.1134/S0026261710030057 Microbiology. New York: Maik Nauka/interperiodica/springer, v. 79, n. 3, p. 306-313, 2010. 0026-2617 http://hdl.handle.net/11449/21588 10.1134/S0026261710030057 WOS:000278407800005 |
Idioma(s) |
eng |
Publicador |
Maik Nauka/interperiodica/springer |
Relação |
Microbiology |
Direitos |
closedAccess |
Palavras-Chave | #pectinase #Thermophilic fungi #Thermomucor #Fermentation #Thermostable enzyme #Polygalacturonase |
Tipo |
info:eu-repo/semantics/article |