Effect of GaAlAs Laser Irradiation on the Epiphyseal Cartilage of Rats


Autoria(s): Cressoni, Marcela Dalla Costa; Khalil Dib Giusti, Helena Hanna; Pião, Antonio Carlos; Carvalho, Rodrigo Leal de Paiva; Anaruma, Carlos Alberto; Casarotto, Raquel Aparecida
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

30/09/2013

20/05/2014

30/09/2013

20/05/2014

01/08/2010

Resumo

Objective: To study the effect of an 830-nm gallium-aluminum-arsenic (GaAlAs) diode laser at two different energy densities (5 and 15 J/cm(2)) on the epiphyseal cartilage of rats by evaluating bone length and the number of chondrocytes and thickness of each zone of the epiphyseal cartilage. Background Data: Few studies have been conducted on the effects of low-level laser therapy on the epiphyseal cartilage at different irradiation doses. Materials and Methods: A total of 30 male Wistar rats with 23 days of age and weighing 90 g on average were randomly divided into 3 groups: control group (CG, no stimulation), G5 group (energy density, 5 J/cm(2)), and G15 group (energy density, 15 J/cm(2)). Laser treatment sessions were administered every other day for a total of 10 sessions. The animals were killed 24 h after the last treatment session. Histological slides of the epiphyseal cartilage were stained with hematoxylin-eosin (HE), photographed with a Zeiss photomicroscope, and subjected to histometric and histological analyses. Statistical analysis was performed using one-way analysis of variance followed by Tukey's post hoc test. All statistical tests were performed at a significance level of 0.05. Results: Histological analysis and x-ray radiographs revealed an increase in thickness of the epiphyseal cartilage and in the number of chondrocytes in the G5 and G15 groups. Conclusion: The 830-nm GaAlAs diode laser, within the parameters used in this study, induced changes in the thickness of the epiphyseal cartilage and increased the number of chondrocytes, but this was not sufficient to induce changes in bone length.

Formato

527-532

Identificador

http://dx.doi.org/10.1089/pho.2009.2572

Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc., v. 28, n. 4, p. 527-532, 2010.

1549-5418

http://hdl.handle.net/11449/20896

10.1089/pho.2009.2572

WOS:000280855300013

Idioma(s)

eng

Publicador

Mary Ann Liebert, Inc.

Relação

Photomedicine and Laser Surgery

Direitos

closedAccess

Tipo

info:eu-repo/semantics/article