The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis


Autoria(s): Carvalho, Camila O.; Chagas, Ana Carolina S.; Cotinguiba, Fernando; Furlan, Maysa; Brito, Luciana G.; Chaves, Francisco C. M.; Stephan, Marilia P.; Bizzo, Humberto R.; Amarante, Alessandro Francisco Talamini do
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

10/02/2012

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The indiscriminate use of anthelmintics has resulted in the establishment of parasite resistance. Thus, this study aimed to evaluate the in vitro antiparasitic effect of plant extracts on Haemonchus contortus in sheep and the in vivo effect on Strongyloides venezuelensis in Rattus norvegicus. The plant extracts from Piper tuberculatum, Lippia sidoides, Mentha piperita, Hura crepitans and Carapa guianensis, produced at different research institutions, were chemically analyzed and evaluated through the egg hatch test (EHT) and larval development test (LDT) in H. contortus. P. tuberculatum (150 and 250 mg kg(-1) of body weight) was evaluated for its anthelmintic action on R. norvegicus experimentally infected with S. venezuelensis. In the EHT, the LC50 and LC90 of the extracts were respectively as follows: 0.031 and 0.09 mg mL(-1) for P. tuberculatum, 0.04 and 0.13 mg mL(-1) for L sidoides, 0.037 and 0.10 mg mL(-1) for M. piperita, 2.16 and 17.13 mg mL(-1) for H. crepitans and 2.03 x 10(-6) and 1.22 x 10(-12) mg mL(-1) for C guianensis. In the LDT, the LC50 and LC90 were respectively: 0.02 and 0.031 mg mL(-1) for P. tuberculatum, 0.002 and 0.04 mg mL(-1) for L. sidoides, 0.018 and 0.03 mg mL(-1) for M. piperita, 0.36 and 0.91 mg mL(-1) for H. crepitans and 17.65 and 1890 mg mL(-1) for C. guianensis. The extract of P. tuberculatum showed the following substances: piperamides as (Z)-piplartine, (E)-piplartine, 8,9-dihydropiplartine, piperine, 10,11-dihydropiperine, 5,6 dihydropiperlongumine and pellitorine. The major compounds of the oils were thymol (76.6%) for L sidoides, menthol (27.5%) for M. piperita and oleic acid (46.8%) for C guianensis. Regarding the in vivo test, neither dose of P. tuberculatum caused any significant reduction (P > 0.05) in worm burden and fecal egg counts compared with the control group. We conclude that the extracts of P. tuberculatum, L sidoides and M. piperita have effective activity when tested in vitro, but the doses of the extract of P. tuberculatum have no effect when employed in in vivo tests. (C) 2011 Elsevier B.V. All rights reserved.

Formato

260-268

Identificador

http://dx.doi.org/10.1016/j.vetpar.2011.07.051

Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 183, n. 3-4, p. 260-268, 2012.

0304-4017

http://hdl.handle.net/11449/18983

10.1016/j.vetpar.2011.07.051

WOS:000299458100009

WOS000299458100009.pdf

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Veterinary Parasitology

Direitos

openAccess

Palavras-Chave #Gastrointestinal nematodes #Folk medicine #Plant extracts #Control
Tipo

info:eu-repo/semantics/article