Muscle-specific growth hormone receptor (GHR) overexpression induces hyperplasia but not hypertrophy in transgenic zebrafish


Autoria(s): Figueiredo, Marcio Azevedo; Mareco, Edson A.; Pai Silva, Maeli Dal; Marins, Luis Fernando
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/06/2012

Resumo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Even though growth hormone (GH) transgenesis has demonstrated potential for improved growth of commercially important species, the hormone excess may result in undesired collateral effects. In this context, the aim of this work was to develop a new model of transgenic zebrafish () characterized by a muscle-specific overexpression of the GH receptor (GHR) gene, evaluating the effect of transgenesis on growth, muscle structure and expression of growth-related genes. In on line of transgenic zebrafish overexpressing GHR in skeletal muscle, no significant difference in total weight in comparison to non-transgenics was observed. This can be explained by a significant reduction in expression of somatotrophic axis-related genes, in special insulin-like growth factor I (IGF-I). In the same sense, a significant increase in expression of the suppressors of cytokine signaling 1 and 3 (SOCS) was encountered in transgenics. Surprisingly, expression of genes coding for the main myogenic regulatory factors (MRFs) was higher in transgenic than non-transgenic zebrafish. Genes coding for muscle proteins did not follow the MRFs profile, showing a significant decrease in their expression. These results were corroborated by the histological analysis, where a hyperplasic muscle growth was observed in transgenics. In conclusion, our results demonstrated that GHR overexpression does not induce hypertrophic muscle growth in transgenic zebrafish probably because of SOCS impairment of the GHR/IGF-I pathway, culminating in IGF-I and muscle proteins decrease. Therefore, it seems that hypertrophy and hyperplasia follow two different routes for entire muscle growth, both of them triggered by GHR activation, but regulated by different mechanisms.

Formato

457-469

Identificador

http://dx.doi.org/10.1007/s11248-011-9546-2

Transgenic Research. Dordrecht: Springer, v. 21, n. 3, p. 457-469, 2012.

0962-8819

http://hdl.handle.net/11449/18611

10.1007/s11248-011-9546-2

WOS:000304097400001

Idioma(s)

eng

Publicador

Springer

Relação

Transgenic Research

Direitos

closedAccess

Palavras-Chave #Transgenic zebrafish #Growth hormone receptor #Insulin-like growth factor I #Suppressors of cytokine signaling #Hyperplasia #Skeletal muscle
Tipo

info:eu-repo/semantics/article