Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas


Autoria(s): Veiga, Luciana CS; Bérgamo, Nádia A; Kowalski, Luiz Paulo; Rogatto, Silvia Regina
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/01/2003

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting probes for chromosomes 5 and 16 were used to evaluate a selected series of HNSCC in which G-banding analysis had shown marker chromosomes. FISH analysis failed to confirm the origin of the marker chromosomes, but four out of five cases showed a significant loss of chromosomes 5. This difference between FISH and G-banding results may reflect the smaller number of metaphase analyzed as well as the criteria adopted for sorting these metaphases. Therefore results obtained solely by G-banding analysis should be considered with caution. Our data confirmed the involvement of chromosome 17 in head and neck squamous cell carcinomas.

Formato

121-128

Identificador

http://dx.doi.org/10.1590/S1415-47572003000200003

Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 26, n. 2, p. 121-128, 2003.

1415-4757

http://hdl.handle.net/11449/17935

10.1590/S1415-47572003000200003

S1415-47572003000200003

S1415-47572003000200003.pdf

Idioma(s)

eng

Publicador

Sociedade Brasileira de Genética

Relação

Genetics and Molecular Biology

Direitos

openAccess

Palavras-Chave #FISH #chromosomal aberrations #head and neck cancer #chromosome 17
Tipo

info:eu-repo/semantics/article