LaTBP1: A Leishmania amazonensis DNA-binding protein that associates in vivo with telomeres and GT-rich DNA using a Myb-like domain
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
15/09/2007
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Resumo |
Different species of Leishmania can cause a variety of medically important diseases, whose control and treatment are still health problems. Telomere binding proteins (TBPs) have potential as targets for anti-parasitic chemotherapy because of their importance for genome stability and cell viability. Here, we describe LaTBP1 a protein that has a Myb-like DNA-binding domain, a feature shared by most double-stranded telomeric proteins. Binding assays using full-length and truncated LaTBP1 combined with spectroscopy analysis were used to map the boundaries of the Myb-like domain near to the protein only tryptophan residue. The Myb-like domain of LaTBP1 contains a conserved hydrophobic cavity implicated in DNA-binding activity. A hypothetical model helped to visualize that it shares structural homology with domains of other Myb-containing proteins. Competition assays and chromatin immunoprecipitation confirmed the specificity of LaTBP1 for telomeric and GT-rich DNAs, suggesting that LaTBP1 is a new TBP. (C) 2007 Elsevier B.V. All rights reserved. |
Formato |
399-409 |
Identificador |
http://dx.doi.org/10.1016/j.abb.2007.06.020 Archives of Biochemistry and Biophysics. New York: Elsevier B.V., v. 465, n. 2, p. 399-409, 2007. 0003-9861 http://hdl.handle.net/11449/17878 10.1016/j.abb.2007.06.020 WOS:000249570100011 |
Idioma(s) |
eng |
Publicador |
Elsevier B.V. |
Relação |
Archives of Biochemistry and Biophysics |
Direitos |
closedAccess |
Palavras-Chave | #double-stranded DNA-binding protein #GT-rich DNA #hydrophobic cavity #leishmania amazonensis #myb-like domain #telomeres |
Tipo |
info:eu-repo/semantics/article |