LaTBP1: A Leishmania amazonensis DNA-binding protein that associates in vivo with telomeres and GT-rich DNA using a Myb-like domain


Autoria(s): Lira, Cristina B. B.; de Siqueira Neto, Jair L.; Khater, Leticia; Cagliari, Thiago C.; Peroni, Luis A.; dos Reis, Jose R. R.; Ramos, Carlos H. I.; Cano, Maria I. N.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

15/09/2007

Resumo

Different species of Leishmania can cause a variety of medically important diseases, whose control and treatment are still health problems. Telomere binding proteins (TBPs) have potential as targets for anti-parasitic chemotherapy because of their importance for genome stability and cell viability. Here, we describe LaTBP1 a protein that has a Myb-like DNA-binding domain, a feature shared by most double-stranded telomeric proteins. Binding assays using full-length and truncated LaTBP1 combined with spectroscopy analysis were used to map the boundaries of the Myb-like domain near to the protein only tryptophan residue. The Myb-like domain of LaTBP1 contains a conserved hydrophobic cavity implicated in DNA-binding activity. A hypothetical model helped to visualize that it shares structural homology with domains of other Myb-containing proteins. Competition assays and chromatin immunoprecipitation confirmed the specificity of LaTBP1 for telomeric and GT-rich DNAs, suggesting that LaTBP1 is a new TBP. (C) 2007 Elsevier B.V. All rights reserved.

Formato

399-409

Identificador

http://dx.doi.org/10.1016/j.abb.2007.06.020

Archives of Biochemistry and Biophysics. New York: Elsevier B.V., v. 465, n. 2, p. 399-409, 2007.

0003-9861

http://hdl.handle.net/11449/17878

10.1016/j.abb.2007.06.020

WOS:000249570100011

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Archives of Biochemistry and Biophysics

Direitos

closedAccess

Palavras-Chave #double-stranded DNA-binding protein #GT-rich DNA #hydrophobic cavity #leishmania amazonensis #myb-like domain #telomeres
Tipo

info:eu-repo/semantics/article