Antioxidant activity of indigo and its preventive effect against ethanol-induced DNA damage in rat gastric mucosa


Autoria(s): Farias-Silva, Elisangela; Cola, Maira; Calvo, Tamara R.; Barbastefano, Victor; Ferreira, Anderson L.; Michelattol, Debora de Paula; Alves de Almeida, Ana Cristina; Hiruma-Lima, Clelia A.; Vilegas, Wagner; Brito, Alba R. M. Souza
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/10/2007

Resumo

Ethanol-induced oxidative damage is commonly associated with the generation of reactive oxygen molecules, leading to oxidative stress. Considering that antioxidant activity is an important mechanism of action involved in cytoprotection, the aim of this work was to evaluate the antioxidant properties of the alkaloid indigo (1) (2 mg/kg, p. o.), obtained from the leaves of Indigofera truxillensis Kunth (Fabaceae), on rat gastric mucosa submitted to ethanol-induced (100%, 1 mL, p.o.) gastric ulcer. Enzymatic assays and DNA fragmentation analysis were performed. When ethanol was administered to the control group, the sulfhydryl content (SH) and the glutathione peroxidase (GPx) activity decreased by 41% and 50%, respectively; in contrast, superoxide dismutase (SOD) and glutathione reductase (GR) activities increased by 56% and 67%, respectively. Additionally, myeloperoxidase (MPO) activity, a marker for free radical generation caused by polymorphonuclear neutrophil (PMN) tissue infiltration, also increased 4.5-fold after ethanol treatment. Rat gastric mucosa exposed to ethanol showed DNA fragmentation. Indigo alkaloid pretreatment protected rats from ethanol-induced gastric lesions. This effect was determined by the ulcerative lesion area (ULA), indicating an inhibition of around 80% at 2 mg/kg. This alkaloid also diminished GPx activity, which was higher than that observed with ethanol alone. However, this effect was counterbalanced by increased GR activity. Indigo was unable to restore alterations in SOD activity promoted by ethanol. After indigo pretreatment, SH levels and MPO activity remained normal and gastric mucosa DNA damage caused by ethanol was also partially prevented by indigo. These results suggest that the gastroprotective mechanisms of indigo include non-enzymatic antioxidant effects and the inhibition of PMN infiltration which, in combination, partially protect the gastric mucosa against ethanol-induced DNA damage.

Formato

1241-1246

Identificador

http://dx.doi.org/10.1055/s-2007-981613

Planta Medica. Stuttgart: Georg Thieme Verlag Kg, v. 73, n. 12, p. 1241-1246, 2007.

0032-0943

http://hdl.handle.net/11449/17727

10.1055/s-2007-981613

WOS:000250481000002

Idioma(s)

eng

Publicador

Georg Thieme Verlag Kg

Relação

Planta Medica

Direitos

closedAccess

Palavras-Chave #alkaloids #Indigofera truxillensis #indigo #Fabaceae #free radicals #antioxidant enzymes #gastric ulcer #MPO activity #DNA damage
Tipo

info:eu-repo/semantics/article