Histological and histomorphometrical evaluation of furcation perforations filled with MTA, CPM and ZOE


Autoria(s): da Silva, G. F.; Guerreiro-Tanomaru, J. M.; Sasso-Cerri, E.; Tanomaru-Filho, M.; Cerri, Paulo Sérgio
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/02/2011

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Processo FAPESP: 04/09898-0

Processo FAPESP: 06/54776-6

P>AimTo evaluate the biological response of the periodontium adjacent to furcation perforations in rat molars filled with Endo-CPM-Sealer (CPM), MTA-Angelus (MTA) or zinc oxide-eugenol cement (ZOE).MethodologyThe pulp chamber floors of maxillary right first molar teeth were perforated and sealed with CPM, mineral trioxide aggregate (MTA) or ZOE; the left first molars, without any treatment, were used as controls (CG). After 7, 15, 30 and 60 days, fragments of maxilla were fixed, decalcified and embedded in paraffin. Sections were stained with H&E, Masson's trichrome and submitted to tartrate-resistant acid phosphatase (TRAP) reaction, used as an osteoclast marker. The width of the periodontal space, the numerical density of inflammatory cells and the number of TRAP-positive osteoclasts in the bone surface were measured, and statistical analyses were performed using analysis of variance and Tukey test (P < 0.05).ResultsIn all experimental groups, the greatest number of inflammatory cells was observed at 7 days, especially in the ZOE group. In this group, the intense inflammatory process was related to a significant increase (P < 0.05) in the number of osteoclasts and, thereby, in an increase in the width of the periodontal space. At 60 days, no significant differences in osteoclast numbers amongst CPM, MTA and CG groups occurred; the periodontal space was also significantly reduced in the experimental groups in comparison with the initial periods. However, in the ZOE group, the periodontal space was significantly larger (P < 0.05) in comparison with MTA-based materials.ConclusionsThe periodontium adjacent to perforations filled with MTA and CPM exhibited clear evidence of re-establishment and thus better biocompatibility than ZOE.

Formato

100-110

Identificador

http://dx.doi.org/10.1111/j.1365-2591.2010.01803.x

International Endodontic Journal. Malden: Wiley-blackwell Publishing, Inc, v. 44, n. 2, p. 100-110, 2011.

0143-2885

http://hdl.handle.net/11449/16773

10.1111/j.1365-2591.2010.01803.x

WOS:000285970800002

Idioma(s)

eng

Publicador

Wiley-Blackwell Publishing, Inc

Relação

International Endodontic Journal

Direitos

closedAccess

Palavras-Chave #biocompatibility #furcal perforations #mineral trioxide aggregate
Tipo

info:eu-repo/semantics/article