Signaling pathways associated with the expression of inflammatory mediators activated during the course of two models of experimental periodontitis


Autoria(s): de Aquino, Sabrina Garcia; Manzolli Leite, Fabio Renato; Stach-Machado, Dagmar Ruth; Francisco da Silva, Juliete Aparecida; Spolidório, Luis Carlos; Rossa, Carlos
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

30/09/2013

20/05/2014

30/09/2013

20/05/2014

22/05/2009

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Processo FAPESP: 05/04428-9

Processo FAPESP: 06/07283-4

Aims: Evaluate the signaling pathways associated with inflammatory mediators activated in two models of experimental periodontitis.Main methods: Two models were used: lipopolysaccharide (LPS) injections and ligature placement. Wistar rats were used and 30 mu g LIPS from Escherichia coli was injected twice a week into the palatal aspect of the upper molars. Ligatures were placed around lower first molars. A control group received injections of PBS on the palatal gingivae whereas no ligatures were placed on the lower molars. Samples were collected 5,15 and 30 days and processed for analysis by Western blotting and stereometry.Key findings: The ligature model was associated with rapid and transient activation of extracellular-regulated kinases (ERK) and p38 mitogen-activated protein kinase (MAPK) as well as of nuclear factor kappa B (NF-kappa B). Activation of these signaling pathways on the LPS model was delayed but sustained throughout the 30-day experimental period. Inflammatory changes induced by both models were similar; however there was a significant reduction on inflammation degree on the ligature model, which paralleled the decrease observed on the activation of the signaling pathways. Activation of signal transducer and activator of transcription (SEAT)-3 by phosphorylation of Tyrosine residues and of SPAT-5 was observed only on the ligature model.Significance: Regulation of gene expression results from the activation of signaling pathways initiated by receptor-ligand binding of external antigens and also of cytokines produced by the host immune system. Understanding the signaling pathways relevant fora given condition may provide information useful for novel therapeutic approaches. (C) 2009 Elsevier B.V. All rights reserved.

Formato

745-754

Identificador

http://dx.doi.org/10.1016/j.lfs.2009.03.001

Life Sciences. Oxford: Pergamon-Elsevier B.V. Ltd, v. 84, n. 21-22, p. 745-754, 2009.

0024-3205

http://hdl.handle.net/11449/15932

10.1016/j.lfs.2009.03.001

WOS:000266281600007

Idioma(s)

eng

Publicador

Pergamon-Elsevier B.V. Ltd

Relação

Life Sciences

Direitos

closedAccess

Palavras-Chave #Cell signaling #Mitogen-activated protein kinase (MAPK) #Chronic inflammation #Periodontal disease #Experimental models
Tipo

info:eu-repo/semantics/article