Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts


Autoria(s): Schwartz-Filho, Humberto Osvaldo; Morandini, Ana Carolina Faria; Ramos-Junior, Erivan Schnaider; Jimbo, Ryo; Santos, Carlos Ferreira; Marcantonio, Elcio; Wennerberg, Ann; Marcantonio, Rosemary Adriana Chierici
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

30/09/2013

20/05/2014

30/09/2013

20/05/2014

01/10/2012

Resumo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Implant topography is an important factor that influences many cell types. To understand the role of topography in the inflammatory events, we evaluated the response of human gingival fibroblasts (HGFs) by the release pattern of cytokines. HGFs were cultured on Ti discs for 24 and 48 h. Four different surface treatments were used: machining method (turned), blasting followed by an acid-etching method (BAE), oxidative nanopatterning (ON) method, and an association of blasting followed by an acid-etching plus oxidative nanopatterning (BAE+ON) method. Extracellular levels of IL-6, IL-8, transforming growth factor beta (TGF-beta), IL-4, and IL-10 were measured by enzyme-linked immunosorbant assay. Increased levels of IL-6 and IL-8 were observed in all surfaces after 24 h which decreased after 48 h. BAE, ON, and BAE+ON surfaces showed a reduction in IL-6 levels compared with the turned after 48 h (p < 0.05). on one hand, IL-8 production was lower in BAE+ON in comparison to the turned surface (p < 0.05). on the other hand, IL-4 showed increased levels with 48 h, which were significantly different between turned, BAE, and on surfaces, but not with BAE+ON. Additionally, TGF-beta and IL-10 production were not detected. This study indicates that nanotopography might be important in the modulation of the inflammatory response in cultured HGFs. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 100A:2629-2636, 2012.

Formato

2629-2636

Identificador

http://dx.doi.org/10.1002/jbm.a.34200

Journal of Biomedical Materials Research Part A. Hoboken: Wiley-blackwell, v. 100A, n. 10, p. 2629-2636, 2012.

1549-3296

http://hdl.handle.net/11449/15922

10.1002/jbm.a.34200

WOS:000307888200011

Idioma(s)

eng

Publicador

Wiley-Blackwell

Relação

Journal of Biomedical Materials Research - Part A

Direitos

closedAccess

Palavras-Chave #cytokines #inflammation #fibroblast #nanotopography #titanium
Tipo

info:eu-repo/semantics/article