Colonisation of soft lining materials by micro-organisms


Autoria(s): Pavan, Sabrina; Santos, Paulo Henrique dos; Arioli Filho, João Neudenir; Palomare Spolidorio, Denise Madalena
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

30/09/2013

20/05/2014

30/09/2013

20/05/2014

01/09/2010

Resumo

Objective:This study evaluated the in vitro adherence of pathogenic micro-organisms, Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa, to soft lining materials and their inhibitory effect on these micro-organisms.Materials and Methods:To measure adherence, specimens of Molloplast B and Ufi Gel P were inoculated [107 colony-forming units per millimetre (cfu/ml)] with TSB media containing the micro-organisms. To determine the number of micro-organisms in the 10-2-10-5 dilutions, 25 mu l of the suspension were transferred to plates of selective media. Colony counts of each specimen were quantified (cfu/ml). The surface roughness was measured with a perfilometer to assess the relationship between the adherence of micro-organisms and surface roughness of each material. For the inhibition test, specimens of materials were placed in agar plates inoculated individually with the micro-organisms. After 48 h, the inhibition zones around the specimens were measured.Results:None of the materials exhibited inhibition zones. The number of cfu/ml of S. aureus and P. aeruginosa were significantly greater than C. albicans for both materials. The Ufi Gel P exhibited greater adherence of C. albicans than Molloplast B. No correlation was observed between the adherence of micro-organisms and surface roughness.Conclusion:The surface roughness of the materials is not the only factor governing micro-organism adherence.

Formato

211-216

Identificador

http://dx.doi.org/10.1111/j.1741-2358.2009.00300.x

Gerodontology. Malden: Wiley-blackwell, v. 27, n. 3, p. 211-216, 2010.

0734-0664

http://hdl.handle.net/11449/15196

10.1111/j.1741-2358.2009.00300.x

WOS:000281209300010

Idioma(s)

eng

Publicador

Wiley-Blackwell

Relação

Gerodontology

Direitos

closedAccess

Palavras-Chave #soft lining materials #micro-organisms #surface roughness #colonisation
Tipo

info:eu-repo/semantics/conferenceObject