Mechanism of calcium hydroxide-induced neutrophil migration into air-pouch cavity
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
30/09/2013
20/05/2014
30/09/2013
20/05/2014
01/06/2008
|
Resumo |
The aim of this study was to investigate cellular migration induced by calcium hydroxide to air-pouch cavities in mice. The migration was more specific to neutrophil and was dose and time dependent (peaking 96 h after stimulation). This migration was inhibited by pretreatment with thalidomide, indomethacin, MK886, meloxicam, dexamethasone, MK886 associated with indomethacin, and MK886 associated with indomethacin and dexamethasone. The air-pouch exudate from animals stimulated with calcium hydroxide showed an increase of leukotriene-B4 (LTB4), interleukin-1 beta, tumor necrosis factor alpha (TNF-alpha), cytokine-induced neutrophil chemoattractant (KC), and macrophage inflammatory protein 2 (MIP-2) release. Pretreatment with 3% thioglycollate increased the macrophage population in the air pouch but did not change neutrophil migration. Depleting the resident mast cells through chronic pretreatment with compound 48/80 did not alter neutrophil migration in response to calcium hydroxide. It was possible to conclude that calcium hydroxide-induced neutrophil migration to the air-pouch cavity in mice is mediated by LTB4, TNF-alpha, KC, MIP-2, and prostaglandins, but it was not dependent on macrophages or mast cells. |
Formato |
814-821 |
Identificador |
http://dx.doi.org/10.1016/j.tripleo.2007.12.013 Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology. New York: Mosby-elsevier, v. 105, n. 6, p. 814-821, 2008. 1079-2104 http://hdl.handle.net/11449/14965 10.1016/j.tripleo.2007.12.013 WOS:000256261800025 |
Idioma(s) |
eng |
Publicador |
Mosby-elsevier |
Relação |
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology |
Direitos |
closedAccess |
Tipo |
info:eu-repo/semantics/article |