Effect of melatonin on DNA damage of bovine cumulus cells during in vitro maturation (IVM) and on in vitro embryo development


Autoria(s): Takada, L.; Martins Júnior, Alicio; Mingoti, Gisele Zoccal; Balieiro, J. C. C.; Cipolla-Neto, J.; Coelho, L. A.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

30/09/2013

20/05/2014

30/09/2013

20/05/2014

01/02/2012

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The effect of melatonin during in vitro maturation (IVM) on DNA damage of cumulus cells (CCs) from bovine cumulus-oocyte complexes (COCs) and embryo development was evaluated. COCs from abattoir ovaries were cultured in maturation medium (MM) with 0.5 mu g/ml FSH and 5.0 mu g/ml LH (FSH-LH); 10(-9) M melatonin (MEL) or FSH-LH + MEL (FSH-LH-MEL). After 24 h of in vitro maturation, the CCs surrounding the oocyte were subjected to DNA analysis by Comet assay. After in vitro fertilization and in vitro embryo culture, the embryo development rates were evaluated on day 2 post insemination (cleavage) and days 7-8 (blastocyst). The percentage of CCs with no DNA damage was significantly superior in MEL group (37.6 +/- 2.4) than in FSH-LH-MEL (28.0 +/- 2.4) and FSH-LH (17.8 +/- 2.41) groups. Cleavage and blastocysts rates were similar among groups. Melatonin during IVM protects the CCs from DNA damage but this effect did not influence embryo development in vitro. (C) 2010 Elsevier Ltd. All rights reserved.

Formato

124-127

Identificador

http://dx.doi.org/10.1016/j.rvsc.2010.11.004

Research In Veterinary Science. Oxford: Elsevier B.V., v. 92, n. 1, p. 124-127, 2012.

0034-5288

http://hdl.handle.net/11449/14839

10.1016/j.rvsc.2010.11.004

WOS:000299578700022

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Research in Veterinary Science

Direitos

closedAccess

Palavras-Chave #Bovine #Comet assay #Cumulus cells #Gonadotropins #In vitro fertilization #Melatonin
Tipo

info:eu-repo/semantics/article