Wild rabies virus detection by plaque assay from naturally infected brains in different species


Autoria(s): Cardoso, T.; Pilz, D.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

26/02/2014

20/05/2014

26/02/2014

20/05/2014

15/11/2004

Resumo

A simple, sensitive and specific plaque assay protocol for the detection of wild type rabies virus in different species is described using confluent monolayers of chicken embryo cells in 6-well plates. Plaques are produced after application of either agarose or Sephadex G-100 overlay onto cell monolayers and incubation for 96 h after virus infection at 37 degreesC. The parameters affecting plaque appearance include cell seeding concentration, overlay composition and time of incubation after infection. Optimal conditions are seeding at a concentration of 4 x 10(6) cell/cm(3), incubation at 37 degreesC in 5% CO2 atmosphere during 96 h, using either 1% agarose or 2% Sephadex G-100 overlays. The described plaque assay would be a new valuable too] in conducting various quantitative investigations, since the chicken embryo cells are susceptible to rabies virus infection from all species studied. (C) 2004 Elsevier B.V. All rights reserved.

Formato

161-167

Identificador

http://dx.doi.org/10.1016/j.vetmic.2004.07.017

Veterinary Microbiology. Amsterdam: Elsevier B.V., v. 103, n. 3-4, p. 161-167, 2004.

0378-1135

http://hdl.handle.net/11449/14729

10.1016/j.vetmic.2004.07.017

WOS:000225111500004

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Veterinary Microbiology

Direitos

closedAccess

Palavras-Chave #rabies virus #plaque assay #chicken embryo related cells
Tipo

info:eu-repo/semantics/article