Virulence genes and plasmid profiles in Rhodococcus equi isolates from domestic pigs and wild boars (Sus scrofa) in Brazil


Autoria(s): Ribeiro, Márcio Garcia; Takai, Shinji; Guazzelli, Alessandro; Batista Lara, Gustavo Henrique; da Silva, Aristeu Vieira; Fernandes, Marta Catarina; Zeni Condas, Larissa Anuska; Siqueira, Amanda Keller; Salerno, Tatiana
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/12/2011

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Processo FAPESP: 06/59298-5

Processo FAPESP: 06/50406-0

The virulence genes and plasmid profiles of 23 Rhodococcus equi isolates from 258 lymph nodes from domestic pigs (129 nodes with lesions and 129 without lesions) and 120 lymph nodes from slaughtered wild boars (60 nodes with lesions and 60 without) were characterized. R. equi was obtained from 19 lymph nodes of domestic pigs, 17 with, and two without lesions, and from four lymph nodes with lesions, from wild boars. The 23 isolates were tested for the presence of vapA and vapB genes, responsible for the 15-17 and 20 kDa virulence-associated proteins, respectively, by PCR in order to characterize as virulent (VapA), intermediately virulent (VapB) and avirulent. Plasmid DNAs were isolated and analyzed by digestion with restriction endonucleases to estimate size and compare their polymorphisms. of the 19 domestic pigs strains, seven (36.8%) were avirulent and 12 (63.2%) were intermediately virulent, with the intermediately virulent isolates being plasmid types 8 (8 isolates), 10 (2 isolates), 1 (1 isolate) and 29 (1 isolate). The plasmid type of four strains isolated from wild boars was also intermediately virulent type 8. None of the domestic pigs and wild boar isolates showed the vapA gene. These findings demonstrate a high occurrence of plasmid type 8 in isolates from pigs and wild boars, and the similarity of plasmid types in the domestic pigs, wild boars and human isolates in Brazil. (C) 2010 Elsevier Ltd. All rights reserved.

Formato

478-481

Identificador

http://dx.doi.org/10.1016/j.rvsc.2010.09.022

Research In Veterinary Science. Oxford: Elsevier B.V., v. 91, n. 3, p. 478-481, 2011.

0034-5288

http://hdl.handle.net/11449/13918

10.1016/j.rvsc.2010.09.022

WOS:000298939200025

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Research in Veterinary Science

Direitos

closedAccess

Palavras-Chave #Pigs #Wild boars #Rhodococcus equi #Virulence #Lymph nodes #Brazil
Tipo

info:eu-repo/semantics/article