Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats
| Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
|---|---|
| Data(s) |
20/05/2014
20/05/2014
01/01/2010
|
| Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Processo FAPESP: 05/03017-5 Processo FAPESP: 06/02860-3 The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR. |
| Formato |
368-374 |
| Identificador |
http://dx.doi.org/10.1590/S1678-91992010000200016 Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 2, p. 368-374, 2010. 1678-9199 http://hdl.handle.net/11449/13887 S1678-91992010000200016 WOS:000278873100016 S1678-91992010000200016-en.pdf |
| Idioma(s) |
eng |
| Publicador |
Universidade Estadual Paulista (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) |
| Relação |
Journal of Venomous Animals and Toxins Including Tropical Diseases |
| Direitos |
openAccess |
| Palavras-Chave | #Toxoplasma gondii #parasite in milk #female rats #PCR #bioassay |
| Tipo |
info:eu-repo/semantics/article |
