STORAGE RESULTS IN LOSS of THE ANTIGENOTOXIC PROPERTIES of LENTINULA EDODES (SHIITAKE MUSHROOM) and DEVELOPMENT of IN VIVO GENOTOXICITY
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/02/2010
|
Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) In this study, we have investigated the stability of the antigenotoxic properties of dried of Lentinula edodes (Berk.) Singer mushroom powder stored at room and at -20C. Mice were pretreated by gavage for 15 consecutive days with 0.6 mL/day of an aqueous extract of dried L. edodes powder prepared at 60C. on day 15, the mice were intraperitoneally injected with 50 mg/kg N-ethyl-N-nitrosourea (ENU); 3 h later, peripheral blood was withdrawn from the animals and used to evaluate DNA damage using the comet assay. The mice were sacrificed 24 h after the ENU treatment and the bone marrow used to measure the frequency of micronucleated polychromatic erythrocytes. The results indicated that storage at both room temperature and at -20C significantly reduced the antigenotoxic effects of the mushroom powder. In addition, whereas freshly dried mushroom powder was nongenotoxic, storage of the powder at -20C resulted in genotoxic activity (P < 0.05). Taken together, our data indicate that constituents of L. edodes powder are altered during storage, resulting in both genotoxicity and a reduction in antigenotoxicity. |
Formato |
206-228 |
Identificador |
http://dx.doi.org/10.1111/j.1745-4514.2009.00275.x Journal of Food Biochemistry. Malden: Wiley-blackwell Publishing, Inc, v. 34, n. 1, p. 206-228, 2010. 0145-8884 http://hdl.handle.net/11449/13030 10.1111/j.1745-4514.2009.00275.x WOS:000274200900016 |
Idioma(s) |
eng |
Publicador |
Wiley-Blackwell Publishing, Inc |
Relação |
Journal of Food Biochemistry |
Direitos |
closedAccess |
Tipo |
info:eu-repo/semantics/article |