Inhibitory effect of silibinin on tumour necrosis factor-alpha and hydrogen peroxide production by human monocytes
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/01/2010
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Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Processo FAPESP: 03/10476-0 Silibinin is a chemically defined flavonoid and the main active component of silymarin, a polyphenolic complex from Silybum marianum, which has anti-inflammatory, hepatoprotective and anticarcinogenic properties. Monocytes obtained from healthy individuals were incubated with silibinin to evaluate cell viability, hydrogen peroxide (H2O2) release and tumour necrosis factor-alpha (TNF-) production by these cells. The duration of treatment and different silibinin concentrations had no significant effect on cell viability. Monocytes showed a dose-dependent inhibitory effect on H2O2 release by phorbol myristate acetate-stimulated monocytes in silibinin concentrations ranging from 6.25 to 50 mu g mL-1. Significant inhibition of TNF- production by lipopolysaccharide-stimulated monocytes was observed at concentrations of 12.5, 50 and 100 mu g mL-1 of silibinin. These results suggest that silibinin exerts antioxidant and anti-inflammatory properties on human monocytes through an inhibitory effect on H2O2 release and on TNF- production, respectively. |
Formato |
1747-1757 |
Identificador |
http://dx.doi.org/10.1080/14786410903314492 Natural Product Research. Abingdon: Taylor & Francis Ltd, v. 24, n. 18, p. 1747-1757, 2010. 1478-6419 http://hdl.handle.net/11449/12185 10.1080/14786410903314492 WOS:000283687900011 |
Idioma(s) |
eng |
Publicador |
Taylor & Francis Ltd |
Relação |
Natural Product Research |
Direitos |
closedAccess |
Palavras-Chave | #hydrogen peroxide #TNF- #flavonoid #silibinin #monocytes |
Tipo |
info:eu-repo/semantics/article |