Combinations of low doses of unfractionated heparin and of low-molecular-weight heparin prevent experimental venous thrombosis


Autoria(s): Carelli, G.; Maffei, Francisco Humberto de Abreu; Mattar, L.; Ferrari, I. C.; Thomazini-Santos, I. A.; de Carvalho, L. R.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/01/2005

Resumo

Synergism between low-molecular-weight heparin and low doses of unfractionated heparin (UH) enhancing anti-factor Xa activity and the release of tissue factor pathway inhibitor was observed. The aim of this study was to verify whether this association is effective in preventing experimental venous thrombosis. Seventy rats were allocated into 7 groups: the control group treated with distilled water, the H-350 group treated with UH 350 IU/kg, the E-2 group treated with enoxaparin 2 mg/kg, the H-175 group treated with UH 175 IU/kg, the E-1 group treated with enoxaparin 1 mg/kg, the H-175 + E-1 group treated with UH 175 IU/kg plus enoxaparin 1 mg/kg, and the H-100 + E-0.5 group treated with UH 100 IU/kg plus enoxaparin 0.5 mg/kg. Forty minutes after subcutaneous injection, thrombosis was induced in vena cava. Three hours later, if present, thrombi were withdrawn and weighed. Bleeding time, activated partial thromboplastin time, thrombin time (TT), and anti-factor Xa were measured at the beginning and end of the experiment. Fortyeight other animals were treated, but without inducing thrombus, and tests were performed 40 min after injection. Thrombus developed in 90.9% of control animals, 20% of the H-350 group, 22.2% of the E-2 group, 10% of the H-175 + E-1 group, and 30% of the H-100 + E-0.5 group; there was a difference between group C and the other groups. Only in the H-350 and H-175 + E-1 groups were TT and activated partial thromboplastin time prolonged in relation to control at the end of the experiment. Forty minutes after injection, TT was prolonged in the H-350 and H-175 + E-1 groups. In conclusion, combinations of low doses of low-molecular-weight heparin and low doses of UH were as effective as high doses of each one used alone in preventing thrombus development in rat vena cava. Copyright (c) 2005 S. Karger AG, Basel.

Formato

263-268

Identificador

http://dx.doi.org/10.1159/000093105

Pathophysiology of Haemostasis and Thrombosis. Basel: Karger, v. 34, n. 6, p. 263-268, 2005.

1424-8832

http://hdl.handle.net/11449/11035

10.1159/000093105

WOS:000238425300003

Idioma(s)

eng

Publicador

Karger

Relação

Pathophysiology of Haemostasis and Thrombosis

Direitos

closedAccess

Palavras-Chave #low-molecular-weight heparin #experimental thrombosis #rat vena cava
Tipo

info:eu-repo/semantics/article