Antioxidant Activity and Total Phenols from the Methanolic Extract of Miconia albicans (Sw.) Triana Leaves


Autoria(s): Pieroni, Lais Goyos; de Rezende, Fernanda Mendes; Ximenes, Valdecir Farias; Dokkedal, Anne Lígia
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/11/2011

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Miconia is one of the largest genus of the Melastomataceae, with approximately 1,000 species. Studies aiming to describe the diverse biological activities of the Miconia species have shown promising results, such as analgesic, antimicrobial and trypanocidal properties. M. albicans leaves were dried, powdered and extracted to afford chloroformic and methanolic extracts. Total phenolic contents in the methanolic extract were determined according to modified Folin-Ciocalteu method. The antioxidant activity was measured using AAPH and DPPH radical assays. Chemical analysis was performed with the n-butanol fraction of the methanolic extract and the chloroformic extract, using different chromatographic techniques (CC, HPLC). The structural elucidation of compounds was performed using 500 MHz NMR and HPLC methods. The methanolic extract showed a high level of total phenolic contents; the results with antioxidant assays showed that the methanolic extract, the n-butanolic fraction and the isolated flavonoids from M. albicans had a significant scavenging capacity against AAPH and DPPH. Quercetin, quercetin-3-O-glucoside, rutin, 3-(E)-p-coumaroyl-alpha-amyrin was isolated from the n-butanolic fraction and a-amyrin, epi-betulinic acid, ursolic acid, epi-ursolic acid from the chloroformic extract. The results presented in this study demonstrate that M. albicans is a promising species in the search for biologically active compounds.

Formato

9439-9450

Identificador

http://dx.doi.org/10.3390/molecules16119439

Molecules. Basel: Mdpi Ag, v. 16, n. 11, p. 9439-9450, 2011.

1420-3049

http://hdl.handle.net/11449/8196

10.3390/molecules16119439

WOS:000297697200040

WOS000297697200040.pdf

Idioma(s)

eng

Publicador

Mdpi Ag

Relação

Molecules

Direitos

openAccess

Palavras-Chave #antioxidant activity #flavonoids #Miconia albicans #total phenolic contents
Tipo

info:eu-repo/semantics/article