Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping


Autoria(s): Miyata, Marcelo; Santos, Adolfo Carlos Barreto; Mendes, Natália Helena; Cunha, Eunice Atsuko; Melo, Fernando Augusto Fiúza de; Leite, Clarice Queico Fujimura
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/06/2011

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.

Formato

774-777

Identificador

http://dx.doi.org/10.1590/S1517-83822011000200045

Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 2, p. 774-777, 2011.

1517-8382

http://hdl.handle.net/11449/7935

10.1590/S1517-83822011000200045

S1517-83822011000200045

WOS:000291614000045

S1517-83822011000200045.pdf

Idioma(s)

eng

Publicador

Sociedade Brasileira de Microbiologia

Relação

Brazilian Journal of Microbiology

Direitos

openAccess

Palavras-Chave #DNA extraction #Mycobacterium tuberculosis #thermolysis #CTAB
Tipo

info:eu-repo/semantics/article