Invasion of epithelial mammalian cells by Paracoccidioides brasiliensis leads to cytoskeletal rearrangement and apoptosis of the host cell


Autoria(s): Mendes-Giannini, Maria José Soares; Hanna, S. A.; Silva, J. L. M. da; Andreotti, P. F.; Vincenzi, L. R.; Benard, G.; Lenzi, H. L.; Soares, Christiane Pienna
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/08/2004

Resumo

Paracoccidioides brasiliensis (Pb) yeast cells can enter mammalian cells and probably manipulate the host cell environment to favor their own growth and survival. We studied the uptake of strain Pb 18 into A549 lung and Vero epithelial cells, with an emphasis on the repercussions in the cytoskeleton and the apoptosis of host cells. Cytoskeleton components of the host cells, such as actin and tubulin, were involved in the P. brasiliensis invasion process. Cytochalasin D and colchicine treatment substantially reduced invasion, indicating the functional participation of microfilaments (MFs) and microtubules (MTs) in this mechanism. Cytokeratin could also play a role in the P. brasiliensis interaction with the host. Gp43 was recognized by anti-actin and anti-cytokeratin antibodies, but not by anti-tubulin. The apoptosis induced by this fungus in infected epithelial cells was demonstrated by various techniques: TUNEL, DNA fragmentation and Bak and Bcl-2 immunocytochemical expression. DNA fragmentation was observed in infected cells but not in uninfected ones, by both TUNEL and gel electrophoresis methods. Moreover, Bcl-2 and Bak did not show any differences until 24 h after infection of cells, suggesting a competitive mechanism that allows persistence of infection. Overexpression of Bak was observed after 48 h, indicating the loss of competition between death and survival signals. In conclusion, the mechanisms of invasion of host cells, persistence within them, and the subsequent induction of apoptosis of such cells may explain the efficient dissemination of P. brasiliensis. (C) 2004 Published by Elsevier SAS.

Formato

882-891

Identificador

http://dx.doi.org/10.1016/j.micinf.2004.05.005

Microbes and Infection. Amsterdam: Elsevier B.V., v. 6, n. 10, p. 882-891, 2004.

1286-4579

http://hdl.handle.net/11449/7674

10.1016/j.micinf.2004.05.005

WOS:000223377200002

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Microbes and Infection

Direitos

closedAccess

Palavras-Chave #Paracoccidioides brasiliensis #cytoskeleton #invasion #apoptosis #host-cell biology
Tipo

info:eu-repo/semantics/article